Document Detail


C312M: an attenuated Vibrio anguillarum strain that induces immunoprotection as an oral and immersion vaccine.
MedLine Citation:
PMID:  23209076     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Vibrio anguillarum, a Gram-negative bacterial pathogen, is the causative agent of vibriosis that affects a wide range of aquatic animals. In this study, we obtained a mutant V. anguillarum, C312M, derived from the pathogenic V. anguillarum C312 by selection of rifampicin resistance. C312M was slower in growth than the wild type C312, particularly under conditions of iron depletion. Compared to C312, C312M was altered in protein production profile and exhibited a dramatically increased median lethal dose. Safety analysis showed that C312M was stable in virulence in the absence of selective pressure. To examine the potential of C312M as a live attenuated vaccine, Japanese flounder Paralichthys olivaceus were vaccinated with C312M via oral, immersion, and oral plus immersion routes. Microbiological analysis showed that C312M was recovered from the gut, liver, kidney, and spleen of the vaccinated fish in 1 to 14 d post-vaccination. When the fish were challenged with C312 at 1 mo post-vaccination, C312M-vaccinated fish exhibited relative percent survival rates of 60 to 84%. Comparable protection was observed when the fish were challenged with a heterologous V. anguillarum strain. Further analysis showed that C312M-vaccinated fish produced specific serum antibodies which enhanced serum bactericidal activity in a manner that is probably complement-dependent. These results indicate that C312M is highly attenuated in virulence but still retains residual infectivity, and that C312M is an effective vaccine when delivered alive via immersion and oral feeding.
Authors:
Lan-Ping Yu; Yong-Hua Hu; Bo-Guang Sun; Li Sun
Related Documents :
24561796 - Identification of vaccine antigens using integrated proteomic analyses of surface immun...
24371566 - Leukocyte transcriptome from chickens infected with avian pathogenic escherichia coli i...
23365206 - Long-term immunogenicity of the pandemic influenza a/h1n1 2009 vaccine among health car...
24667706 - A mouse model for betacoronavirus subgroup 2c using a bat coronavirus strain hku5 variant.
1431706 - Factors influencing childhood immunisation in an urban area of brazil.
21320546 - A comparative review on european-farmed finfish rna viruses and their vaccines.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Diseases of aquatic organisms     Volume:  102     ISSN:  0177-5103     ISO Abbreviation:  Dis. Aquat. Org.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807037     Medline TA:  Dis Aquat Organ     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  33-42     Citation Subset:  IM    
Affiliation:
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, PR China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cohort study of effect of vaccination on pancreas disease in Norwegian salmon aquaculture.
Next Document:  Comparison of genetic characteristics and pathogenicity of Lactococcus garvieae isolated from aquati...