Document Detail

C3 synthesis and CRs expression during differentiation of a murine stem cell line.
MedLine Citation:
PMID:  10776797     Owner:  NLM     Status:  MEDLINE    
C3 production, release and CRs expression during the neutrophilic differentiation of a murine non tumorigenic cell line is investigated. The murine non tumorigenic cell line 32DCl3(G) which undergoes terminal differentiation into polymorphonuclear granulocytes when cultured in presence of G-CSF was selected as a suitable in vitro model for this study. The results show that as the cells progress into the differentiation program, levels of C3 mRNA increase, accompanied by increased C3 production. As differentiation progresses the cells gradually express CRs on their surface; these are undetectable on the surface of undifferentiated cells. As a consequence of CRs appearance, cells become able to bind C3 through receptorial binding. Differences were found in the modality of C3 secretion: differentiated cells tend to store C3 in their intracellular compartments rather than secrete it continuously into the medium and they respond to membrane stimulation with increased secretion of C3. Treatment of 32DCl3(G) with TNF-alpha increased C3 production in a time- and dose-dependent fashion. Cell response to this stimulus progressively increases during the differentiation process suggesting that they acquire functionality in the signal transduction mechanisms.
S Mardente; A Longo; L Lenti; G De Capua; W M Prodinger; I Silvestri; G Pontieri; M Lipari
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunobiology     Volume:  201     ISSN:  0171-2985     ISO Abbreviation:  Immunobiology     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-07-27     Completed Date:  2000-07-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8002742     Medline TA:  Immunobiology     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  420-31     Citation Subset:  IM    
Department of Experimental Medicine and Pathology, University La Sapienza Rome, Italy.
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MeSH Terms
Calcimycin / pharmacology
Cell Differentiation
Cell Line
Complement C3 / biosynthesis*,  genetics
Cytochalasin D / pharmacology
Dose-Response Relationship, Drug
Mice, Inbred C57BL
Receptors, Complement / biosynthesis*
Stem Cells / cytology,  drug effects,  immunology*
Tetradecanoylphorbol Acetate / pharmacology
Time Factors
Tumor Necrosis Factor-alpha / pharmacology
Reg. No./Substance:
0/Complement C3; 0/Receptors, Complement; 0/Tumor Necrosis Factor-alpha; 16561-29-8/Tetradecanoylphorbol Acetate; 22144-77-0/Cytochalasin D; 52665-69-7/Calcimycin

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