Document Detail


C1qTNF-related protein-6 increases the expression of interleukin-10 in macrophages.
MedLine Citation:
PMID:  20652496     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
C1qTNF-Related proteins (CTRPs), a new highly conserved family of adiponectin paralogs, were recently identified as being involved in diverse processes including metabolism, host defense, apoptosis, cell differentiation, and organogenesis. However, the functional role of CTRP6 remains poorly identified. Here we provide evidence that CTRP6 induces the expression of interleukin-10 (IL-10) in macrophages. Conditioned medium from CTRP6-expressing HEK293 cells increased IL-10 expression in Raw264.7 cells. The globular domain of CTRP6 (gCTRP6) also dose-dependently increased both IL-10 mRNA and protein expression levels, with transcript levels increasing within 2 h. Furthermore, the globular domain of CTRP6 rapidly induced phosphorylation of ERK1/2 in Raw264.7 cells. Treatment with U0126, a selective inhibitor, abolished CTRP6-stimulated IL-10 induction. Taken together, there results demonstrate that CTRP6 induces expression of IL-10 via ERK1/2 activation. Considering that IL-10 is a potent anti-inflammatory cytokine that modulates inflammatory signaling pathways, CTRP6 may be a novel target for pharmacological drugs in inflammatory diseases.
Authors:
Mi-Jin Kim; Wan Lee; Eun-Ju Park; Seung-Yoon Park
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-14
Journal Detail:
Title:  Molecules and cells     Volume:  30     ISSN:  0219-1032     ISO Abbreviation:  Mol. Cells     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-11-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9610936     Medline TA:  Mol Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  59-64     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, School of Medicine, Dongguk University, Kyungju 780-714, Korea.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
HEK293 Cells
Humans
Interleukin-10 / biosynthesis*
MAP Kinase Signaling System
Macrophages / metabolism*,  physiology
Mitogen-Activated Protein Kinase 1 / metabolism
Mitogen-Activated Protein Kinase 3 / metabolism
Phosphorylation
Proteins / genetics,  metabolism*
Signal Transduction
Transfection
Chemical
Reg. No./Substance:
0/C1QTNF1 protein, human; 0/Proteins; 130068-27-8/Interleukin-10; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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