Document Detail

C-type natriuretic peptide relaxes human coronary artery bypass grafts preconstricted by endothelin-1.
MedLine Citation:
PMID:  16181867     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Endothelin is implicated in graft spasm after coronary artery bypass grafting. We assessed reversal by the endothelium-derived vasodilator C-type natriuretic peptide of prior contraction of radial artery and other vessels commonly used for coronary artery bypass surgery. METHODS: Segments of human radial artery, saphenous vein, and internal mammary artery were mounted in organ baths after removal from patients undergoing cardiac surgery (n = 34; 64 +/- 2 years). Effects of increasing concentrations of C-type natriuretic peptide (with or without aprotinin, 1,000 U/mL) on endothelin-induced contraction were compared with acetylcholine, sodium nitroprusside, and papaverine. RESULTS: C-type natriuretic peptide relaxed endothelin precontraction in all vessels (F = 17.8, 36.3, and 48.4, respectively; p < 0.001), with maximum relaxations of 44%, 54%, and 66% in saphenous vein, internal mammary artery, and radial artery, respectively. Aprotinin did not affect relaxation to C-type natriuretic peptide. Acetylcholine relaxed the saphenous vein weakly, with maximal relaxation of 9% at 10(-6) M. However, the radial artery and internal mammary artery relaxed strongly to acetylcholine. The highest concentration of papaverine completely relaxed all vessels, but responses were less sensitive than to sodium nitroprusside or acetylcholine. CONCLUSIONS: C-type natriuretic peptide reverses endothelin-induced constriction in arterial and venous conduits used for coronary artery bypass, particularly the radial artery. Proteolytic breakdown of C-type natriuretic peptide by local vascular enzymes appears of little importance in vitro. This signals the therapeutic potential of using C-type natriuretic peptide as an antagonist of graft vasospasm after coronary artery bypass surgery.
Christopher J Kelsall; Adrian H Chester; Mohammed Amrani; Donald R J Singer
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  80     ISSN:  1552-6259     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-26     Completed Date:  2006-09-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1347-51     Citation Subset:  AIM; IM    
Department of Cardiothoracic Surgery, Heart Science Centre and Harefield Hospital, Harefield, Middlesex, United Kingdom.
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MeSH Terms
Acetylcholine / pharmacology
Coronary Artery Bypass*
Coronary Vasospasm / prevention & control*
Dose-Response Relationship, Drug
Endothelin-1 / antagonists & inhibitors*
Mammary Arteries / drug effects
Middle Aged
Natriuretic Agents / pharmacology*
Natriuretic Peptide, C-Type / pharmacology*
Papaverine / pharmacology
Radial Artery / drug effects
Saphenous Vein / drug effects
Treatment Outcome
Vasoconstriction / drug effects
Vasodilator Agents / pharmacology
Reg. No./Substance:
0/Endothelin-1; 0/Natriuretic Agents; 0/Vasodilator Agents; 127869-51-6/Natriuretic Peptide, C-Type; 51-84-3/Acetylcholine; 58-74-2/Papaverine
Comment In:
Ann Thorac Surg. 2005 Oct;80(4):1351-2   [PMID:  16181868 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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