Document Detail


C-type natriuretic peptide regulates blood-testis barrier dynamics in adult rat testes.
MedLine Citation:
PMID:  17360440     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In adult rat testes, the blood-testis barrier (BTB) in the seminiferous epithelium must "open" (or "disassemble") to accommodate the migration of preleptotene spermatocytes from the basal to the adluminal compartment that occurs at stage VIII of the epithelial cycle. However, the molecule(s) and/or mechanism(s) that regulate this event are unknown. In this report, C-type natriuretic peptide (CNP) was shown to be a regulator of BTB dynamics. Although Sertoli and germ cells contributed to the pool of CNP in the seminiferous epithelium, its receptor, natriuretic peptide receptor B, resided almost exclusively in Sertoli cells. CNP also expressed stage-specifically and localized predominantly at the BTB in the seminiferous epithelium at stage VIII of the epithelial cycle. A synthetic CNP-22 peptide, when added to Sertoli cell cultures, was shown to perturb Sertoli cell tight junction in vitro, causing disappearance of BTB-associated proteins (JAM-A, occludin, N-cadherin, and beta-catenin) from the cell-cell interface. This inhibitory effect of CNP on the tight junction was confirmed by transient overexpression of CNP in these cells, which was mediated, at least in part, by accelerating the internalization of BTB integral membrane proteins. To validate these in vitro findings, CNP-22 was administered to testes at a dose of 0.35 or 3.5 mug per testis, which was shown to perturb the BTB integrity In vivo when the barrier function was assessed by monitoring the diffusion of a small molecular probe across the BTB. In summary, CNP secreted by Sertoli and germ cells into the BTB microenvironment regulates BTB dynamics during spermatogenesis.
Authors:
Weiliang Xia; Dolores D Mruk; C Yan Cheng
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-02-27
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  104     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-15     Completed Date:  2007-04-24     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3841-6     Citation Subset:  IM    
Affiliation:
Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10021.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood-Testis Barrier / physiology*
Cell Communication
Endocytosis
Male
Models, Biological
Natriuretic Agents / pharmacology
Natriuretic Peptide, C-Type / physiology*
Rats
Seminiferous Epithelium / metabolism
Sertoli Cells / metabolism
Spermatocytes / metabolism
Spermatogenesis*
Testis / metabolism*
Tight Junctions
Grant Support
ID/Acronym/Agency:
U01 HD 045908/HD/NICHD NIH HHS; U01 HD045908/HD/NICHD NIH HHS; U01 HD045908-01/HD/NICHD NIH HHS; U01 HD045908-02/HD/NICHD NIH HHS; U01 HD045908-03/HD/NICHD NIH HHS; U01 HD045908-04/HD/NICHD NIH HHS; U01 HD045908-05/HD/NICHD NIH HHS; U54 HD 029990/HD/NICHD NIH HHS; U54 HD029990/HD/NICHD NIH HHS; U54 HD029990-110007/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Natriuretic Agents; 127869-51-6/Natriuretic Peptide, C-Type
Comments/Corrections

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