Document Detail


C-terminus of mitotic centromere-associated kinesin (MCAK) inhibits its lattice-stimulated ATPase activity.
MedLine Citation:
PMID:  15250824     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mitotic centromere-associated kinesin (MCAK) is a microtubule (MT)-destabilizing molecular motor. In the present study we show that the final 8 amino acids of the C-terminus of MCAK inhibit lattice-stimulated ATPase activity of the motor. Surprisingly, loss of this C-terminal 'tail' (MCAK-Q710) leads to more rapid depolymerization of MTs relative to full-length MCAK (wt-MCAK). Biochemical and microscopic assays revealed that MCAK-Q710 bound to the MT lattice with higher apparent affinity as compared with wt-MCAK. End-stimulated depolymerization was similar for both enzymes. These data suggest that lattice-bound MCAK can increase the rate of MT depolymerization, but at an energy cost. The function of the C-terminus of MCAK may be to selectively inhibit lattice-stimulated ATPase activity, resulting in limited interactions of the motor with the MT lattice. This increases the coupling between ATP hydrolysis and tubulin dimer release, but it also limits MT depolymerization.
Authors:
Ayana Moore; Linda Wordeman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Biochemical journal     Volume:  383     ISSN:  1470-8728     ISO Abbreviation:  Biochem. J.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-10-07     Completed Date:  2005-03-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  227-35     Citation Subset:  IM    
Affiliation:
Department of Physiology and Biophysics, University of Washington School of Medicine, 1959 NE Pacific St., Box 357290, Seattle, WA 98195, USA. atmoore@u.washington.edu
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Animals
CHO Cells
Cattle
Cells, Cultured
Centromere / metabolism
Cricetinae
Dimerization
Hydrolysis
Kinesin / chemistry*,  genetics,  metabolism*
Kinetics
Microtubules / chemistry,  metabolism
Rhodamines / metabolism
Sequence Deletion
Tubulin / chemistry,  metabolism
Grant Support
ID/Acronym/Agency:
1 F31 GM65061/GM/NIGMS NIH HHS; GM53654A/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Rhodamines; 0/Tubulin; 0/mitotic centromere-associated kinesin, hamster; 56-65-5/Adenosine Triphosphate; EC 3.6.1.-/Kinesin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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