| C-terminal residues 621-635 of protein S are essential for binding to factor Va. | |
| | |
MedLine Citation:
|
PMID: 10593904 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Protein S is anticoagulant in the absence of activated protein C because of direct interactions with coagulation Factors Xa and Va. Synthetic peptides corresponding to amino acid sequences of protein S were tested for their ability to inhibit prothrombinase activity. The peptide containing the C-terminal sequence of protein S, residues 621-635 (PSP14), reversibly inhibited prothrombinase activity in the presence but not in the absence of Factor Va (K(i) approximately 2 microM). PSP14 inhibition of prothrombinase was independent of phospholipids but could be competitively overcome by increasing Factor Xa concentrations, suggesting that the C-terminal region of protein S may compete for a Factor Xa binding site on Factor Va. Studies using peptides with amino acid substitutions suggested that lysines 630, 631, and 633 were critical residues. PSP14 inhibited Factor Va activity in Factor Xa-one-stage clotting assays. PSP14 inhibited protein S binding to immobilized Factor Va. When preincubated with protein S, antibodies raised against PSP14 inhibited binding of protein S to Factor Va and blocked inhibition of prothrombinase activity by protein S. These results show that the C-terminal region of protein S containing residues 621-635 is essential for binding of protein S to Factor Va and that this interaction contributes to anticoagulant action. |
| | |
Authors:
|
M J Heeb; Y Kojima; J Rosing; G Tans; J H Griffin |
Related Documents
:
|
23102234 - Three dimensional printing of soy protein scaffolds for tissue regeneration. 3810664 - Comparison of immunological, biochemical and biophysical properties of three hemorrhagi... 22952844 - Targeted ubiquitination and degradation of g-protein-coupled receptor kinase 5 by the d... 23467414 - Class iia histone deacetylases and mef2 proteins regulate the mesenchymal-to-epithelial... 21412434 - Tdp-43 regulates drosophila neuromuscular junctions growth by modulating futsch/map1b l... 7274294 - Nature of copper and zinc compounds in tissues from a patient with menkes kinky hair sy... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 274 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1999 Dec |
Date Detail:
|
Created Date: 2000-01-27 Completed Date: 2000-01-27 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 36187-92 Citation Subset: IM |
Affiliation:
|
Departments of Molecular and Experimental Medicine and Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA. heeb@scripps.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Binding Sites Blood Coagulation Factor Va / chemistry, metabolism* Humans Molecular Sequence Data Peptide Fragments / chemistry, metabolism Protein Binding Protein S / chemistry, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
R01HL21544/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Peptide Fragments; 0/Protein S; 65522-14-7/Factor Va |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Diacylglycerol kinase epsilon, but not zeta, selectively removes polyunsaturated diacylglycerol, ind...
Next Document: Correction of aberrant splicing of the cystic fibrosis transmembrane conductance regulator (CFTR) ge...