Document Detail


The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidC.
MedLine Citation:
PMID:  22950014     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Type VI secretion systems (T6SS) are macromolecular complexes present in Gram-negative bacteria. T6SS are structurally similar to the bacteriophage cell-puncturing device and have been shown to mediate bacteria-host or bacteria-bacteria interactions. T6SS assemble from 13 to 20 proteins. In enteroaggregative Escherichia coli (EAEC), one of the subassemblies is composed of four proteins that form a trans-envelope complex: the TssJ outer membrane lipoprotein, the peptidoglycan-anchored inner membrane TagL protein, and two putative inner membrane proteins, TssL and TssM. In this study, we characterized the TssL protein of the EAEC Sci-1 T6SS in terms of localization, topology, and function. TssL is a critical component of the T6SS, anchored to the inner membrane through a single transmembrane segment located at the extreme C-terminus of the protein. We further show that this transmembrane segment is essential for the function of the protein and its proper insertion in the inner membrane is dependent upon YidC and modulated by the Hsp70 homologue DnaK.
Authors:
Marie-Stéphanie Aschtgen; Abdelrahim Zoued; Roland Lloubès; Laure Journet; Eric Cascales
Related Documents :
24089364 - Molecular recognition of a model globular protein apomyoglobin by synthetic receptor cy...
23741014 - Two-colored fluorescence correlation spectroscopy screening for lc3-p62 interaction inh...
23892754 - An extracellular serine/threonine-rich protein from lactobacillus plantarum ncimb 8826 ...
3630544 - Influence of steroid hormones on the incorporation of amino acids in uterine and cervic...
15805494 - Functional insights from the distribution and role of homopeptide repeat-containing pro...
18348704 - A protein map and its application.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  MicrobiologyOpen     Volume:  1     ISSN:  2045-8827     ISO Abbreviation:  Microbiologyopen     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-09-05     Completed Date:  2012-09-11     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  101588314     Medline TA:  Microbiologyopen     Country:  England    
Other Details:
Languages:  eng     Pagination:  71-82     Citation Subset:  -    
Affiliation:
Laboratoire d'Ingénierie des Systèmes Macromoléculaires, Institut de Microbiologie de la Méditerranée, Aix-Marseille Université CNRS - UMR 7255, 31 chemin Joseph Aiguier, 13402, Marseille Cedex 20, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Comparison of Scheffersomyces stipitis strains CBS 5773 and CBS 6054 with regard to their xylose met...
Next Document:  Eukaryotic life in biofilms formed in a uranium mine.