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C-reactive protein enhances the respiratory burst of neutrophils-induced by antineutrophil cytoplasmic antibody.
MedLine Citation:
PMID:  22683644     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Serum C-reactive protein (CRP) was one of the useful biomarkers for evaluating the disease activity in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). Cumulating studies proved that CRP was pathogenic in a variety of diseases. In the current study, the in vitro effects of CRP to prime neutrophils for ANCA-induced respiratory burst were investigated with flow cytometry. Without TNF-α in the reactive system, ANCA could only induce a slight level of respiratory burst of neutrophils. CRP could enhance the respiratory burst of neutrophils induced by ANCA against myeloperoxidse [mean fluorescence intensity (MFI, 68.45±16.87 vs. 58.65±15.09, P<0.05) or by ANCA against proteinase 3 (MFI, 79.51±15.90 vs. 61.73±14.89, P<0.05). Although CRP (50μg/mL, incubating for 30min) could not active neutrophils alone, after incubation with neutrophils for 10min, CRP (50μg/mL) could increase the expression of membrane proteinase 3 of neutrophils (MFI, 365.27±143.50 vs. 235.32±124.65, P<0.05). Heat-treated CRP could not enhance the levels of neutrophils respiratory burst induced by ANCA or increase the expression of membrane proteinase 3 of neutrophils. So CRP can prime neutrophils and enhance the respiratory burst induced by ANCA and might be pathogenic in AAV.
Authors:
Peng-Cheng Xu; Jian Hao; Xiao-Wei Yang; Chang Dong-Yuan; Min Chen; Ming-Hui Zhao
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-6
Journal Detail:
Title:  Molecular immunology     Volume:  52     ISSN:  1872-9142     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905289     Medline TA:  Mol Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  148-154     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Renal Division, Department of Medicine, Peking University First Hospital, Beijing 100034, China; Institute of Nephrology, Peking University, Beijing 100034, China; Key Laboratory of Renal Disease, Ministry of Health of China, Beijing 100034, China; Key Laboratory of CKD, Prevention and Treatment, Ministry of Education of China, Beijing 100034, China; Department of Nephrology, General Hospital of Tianjin Medical University, Tianjin 300052, China.
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