| C-jun N-terminal kinase-mediated signaling is essential for Staphylococcus aureus-induced U937 apoptosis. | |
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MedLine Citation:
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PMID: 19382420 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the effect of SP600125, a specific c-jun N-terminal protein kinase (JNK) inhibitor, on Staphylococcus aureus (S. aureus)-induced U937 cell death and the underlying mechanism. METHODS: The human monocytic U937 cells were treated with S. aureus at different time with or without SP600125. Cell apoptosis was analyzed by flow cytometry. JNK, Bax, and caspase-3 activities were detected by Western blotting. RESULTS: S. aureus induced apoptosis in cultured U937 cells in a time-dependent manner. Expression of Bax and phospho-JNK significantly increased in S. aureus-treated U937 cells, and the level of activated caspase-3 also increased in a time-dependent manner. Inhibition of JNK with SP600125 significantly inhibited S. aureus-induced apoptosis in U937 cells. CONCLUSIONS: S. aureus can induce apoptosis in U937 cells by phosphorylation of JNK and activation of Bax and caspase-3. SP600125 protects U937 cells from apoptosis induced by S. aureus via inhibiting the activity of JNK. |
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Authors:
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Jia-he Wang; Bo Yu; Hui-yan Niu; Hui Li; Yi Zhang; Xin Wang; Ping He |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Chinese medical sciences journal = Chung-kuo i hsüeh k'o hsüeh tsa chih / Chinese Academy of Medical Sciences Volume: 24 ISSN: 1001-9294 ISO Abbreviation: Chin. Med. Sci. J. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-04-22 Completed Date: 2009-08-25 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9112559 Medline TA: Chin Med Sci J Country: China |
Other Details:
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Languages: eng Pagination: 26-9 Citation Subset: IM |
Affiliation:
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Department of Geriatrics, Shengjing Hospital, China Medical University, Shenyang 110004, China. cmuwjh@126.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anthracenes
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pharmacology Apoptosis / physiology* Caspase 3 / metabolism Humans JNK Mitogen-Activated Protein Kinases / metabolism* Macrophages / cytology, metabolism, microbiology Mitogen-Activated Protein Kinase 8 / antagonists & inhibitors, metabolism Mitogen-Activated Protein Kinase 9 / antagonists & inhibitors, metabolism Phosphorylation / drug effects Protein Kinase Inhibitors / pharmacology Signal Transduction / physiology* Staphylococcus aureus / physiology* U937 Cells bcl-2-Associated X Protein / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anthracenes; 0/BAX protein, human; 0/Protein Kinase Inhibitors; 0/anthra(1,9-cd)pyrazol-6(2H)-one; 0/bcl-2-Associated X Protein; EC 2.7.1.24/Mitogen-Activated Protein Kinase 9; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinase 8; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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