| The C. elegans developmental fusogen EFF-1 mediates homotypic fusion in heterologous cells and in vivo. | |
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MedLine Citation:
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PMID: 17011487 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During cell-cell fusion, two cells' plasma membranes merge, allowing the cytoplasms to mix and form a syncytium. Little is known about the mechanisms of cell fusion. Here, we asked whether eff-1, shown previously to be essential for fusion in Caenorhabditis elegans, acts directly in the fusion machinery. We show that expression of EFF-1 transmembrane protein drives fusion of heterologous cells into multinucleate syncytia. We obtained evidence that EFF-1-mediated fusion involves a hemifusion intermediate characterized by membrane mixing without cytoplasm mixing. Furthermore, syncytiogenesis requires EFF-1 in both fusing cells. To test whether this mechanism also applies in vivo, we conducted genetic mosaic analysis of C. elegans and found that homotypic epidermal fusion requires EFF-1 in both cells. Thus, although EFF-1-mediated fusion shares characteristics with viral and intracellular fusion, including an apparent hemifusion step, it differs from these reactions in the homotypic organization of the fusion machinery. |
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Authors:
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Benjamin Podbilewicz; Evgenia Leikina; Amir Sapir; Clari Valansi; Meital Suissa; Gidi Shemer; Leonid V Chernomordik |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Developmental cell Volume: 11 ISSN: 1534-5807 ISO Abbreviation: Dev. Cell Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-10-02 Completed Date: 2006-11-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101120028 Medline TA: Dev Cell Country: United States |
Other Details:
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Languages: eng Pagination: 471-81 Citation Subset: IM |
Affiliation:
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Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel. podbilew@tx.technion.ac.il |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified Caenorhabditis elegans / cytology*, embryology*, genetics, metabolism* Caenorhabditis elegans Proteins / genetics, metabolism* Cell Fusion Cell Membrane / metabolism, physiology* Cells, Cultured Embryo, Nonmammalian Genes, Reporter Green Fluorescent Proteins / metabolism Kinetics Membrane Glycoproteins / genetics, metabolism* Models, Biological Mosaicism Spodoptera / cytology Time Factors Transfection Transgenes |
| Chemical | |
Reg. No./Substance:
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0/Caenorhabditis elegans Proteins; 0/EFF-1 protein, C elegans; 0/Membrane Glycoproteins; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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