Document Detail


The C. elegans developmental fusogen EFF-1 mediates homotypic fusion in heterologous cells and in vivo.
MedLine Citation:
PMID:  17011487     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During cell-cell fusion, two cells' plasma membranes merge, allowing the cytoplasms to mix and form a syncytium. Little is known about the mechanisms of cell fusion. Here, we asked whether eff-1, shown previously to be essential for fusion in Caenorhabditis elegans, acts directly in the fusion machinery. We show that expression of EFF-1 transmembrane protein drives fusion of heterologous cells into multinucleate syncytia. We obtained evidence that EFF-1-mediated fusion involves a hemifusion intermediate characterized by membrane mixing without cytoplasm mixing. Furthermore, syncytiogenesis requires EFF-1 in both fusing cells. To test whether this mechanism also applies in vivo, we conducted genetic mosaic analysis of C. elegans and found that homotypic epidermal fusion requires EFF-1 in both cells. Thus, although EFF-1-mediated fusion shares characteristics with viral and intracellular fusion, including an apparent hemifusion step, it differs from these reactions in the homotypic organization of the fusion machinery.
Authors:
Benjamin Podbilewicz; Evgenia Leikina; Amir Sapir; Clari Valansi; Meital Suissa; Gidi Shemer; Leonid V Chernomordik
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental cell     Volume:  11     ISSN:  1534-5807     ISO Abbreviation:  Dev. Cell     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-10-02     Completed Date:  2006-11-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101120028     Medline TA:  Dev Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  471-81     Citation Subset:  IM    
Affiliation:
Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel. podbilew@tx.technion.ac.il
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Caenorhabditis elegans / cytology*,  embryology*,  genetics,  metabolism*
Caenorhabditis elegans Proteins / genetics,  metabolism*
Cell Fusion
Cell Membrane / metabolism,  physiology*
Cells, Cultured
Embryo, Nonmammalian
Genes, Reporter
Green Fluorescent Proteins / metabolism
Kinetics
Membrane Glycoproteins / genetics,  metabolism*
Models, Biological
Mosaicism
Spodoptera / cytology
Time Factors
Transfection
Transgenes
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/EFF-1 protein, C elegans; 0/Membrane Glycoproteins; 147336-22-9/Green Fluorescent Proteins

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