Document Detail


A Candida albicans temperature-sensitive cdc12-6 mutant identifies roles for septins in selection of sites of germ tube formation and hyphal morphogenesis.
MedLine Citation:
PMID:  22886998     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Septins were identified for their role in septation in Saccharomyces cerevisiae and were subsequently implicated in other morphogenic processes. To study septins in Candida albicans hyphal morphogenesis, a temperature-sensitive mutation was created that altered the C terminus of the essential Cdc12 septin. The cdc12-6 cells grew well at room temperature, but at 37°C they displayed expected defects in septation, nuclear localization, and bud morphogenesis. Although serum stimulated the cdc12-6 cells at 37°C to form germ tube outgrowths, the mutant could not maintain polarized hyphal growth and instead formed chains of elongated cell compartments. Serum also stimulated the cdc12-6 mutant to induce a hyphal reporter gene (HWP1-GFP) and a characteristic zone of filipin staining at the leading edge of growth. Interestingly, cdc12-6 cells shifted to 37°C in the absence of serum gradually displayed enriched filipin staining at the tip, which may be due to the altered cell cycle regulation. A striking difference from the wild type was that the cdc12-6 cells frequently formed a second germ tube in close proximity to the first. The mutant cells also failed to form the diffuse band of septins at the base of germ tubes and hyphae, indicating that this septin band plays a role in preventing proximal formation of germ tubes in a manner analogous to bud site selection. These studies demonstrate that not only are septins important for cytokinesis, but they also promote polarized morphogenesis and selection of germ tube sites that may help disseminate an infection in host tissues.
Authors:
Lifang Li; Chengda Zhang; James B Konopka
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-08-10
Journal Detail:
Title:  Eukaryotic cell     Volume:  11     ISSN:  1535-9786     ISO Abbreviation:  Eukaryotic Cell     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-02     Completed Date:  2013-02-21     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101130731     Medline TA:  Eukaryot Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1210-8     Citation Subset:  IM    
Affiliation:
Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Candida albicans / cytology,  genetics*,  growth & development
Cell Cycle Proteins / genetics,  physiology*
Cytokinesis / genetics
Fungal Proteins / genetics,  physiology*
Hyphae / genetics,  growth & development*
Molecular Sequence Data
Morphogenesis / genetics
Mutation
Septins / genetics,  physiology*
Temperature
Grant Support
ID/Acronym/Agency:
R01 AI047837/AI/NIAID NIH HHS; R01 AI47837/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Fungal Proteins; EC 3.6.1.-/Septins
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