Document Detail


The C-terminal domains of adenovirus serotype 5 protein IX assemble into an antiparallel structure on the facets of the capsid.
MedLine Citation:
PMID:  19004948     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adenovirus serotype 5 protein IX (pIX) has two domains connected by a flexible linker. Three N-terminal domains form triskelions on the capsid facets that cement hexons together, and the C-terminal domains of four monomers form complexes toward the facet periphery. Here we present a cryoelectron microscopy structure of recombinant adenovirus with a peptide tag added to the C terminus of pIX. The structure, made up by several C termini of pIX, is longer at both ends than the wild-type protein, and Fabs directed against the tag bind to both ends of the oligomer, demonstrating that the pIX C termini associate in an antiparallel manner.
Authors:
Céline M S Fabry; Manuel Rosa-Calatrava; Christine Moriscot; Rob W H Ruigrok; Pierre Boulanger; Guy Schoehn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-12
Journal Detail:
Title:  Journal of virology     Volume:  83     ISSN:  1098-5514     ISO Abbreviation:  J. Virol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-30     Completed Date:  2009-01-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1135-9     Citation Subset:  IM    
Affiliation:
Université Joseph Fourier, Unit for Virus Host Cell Interaction, UMR 5233 UJF-EMBL-CNRS, Grenoble cedex 9, France.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / chemistry,  physiology*,  ultrastructure*
Antibodies, Viral / metabolism
Capsid Proteins / chemistry,  metabolism*,  ultrastructure*
Cryoelectron Microscopy
Humans
Models, Molecular
Protein Binding
Protein Structure, Quaternary
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/Capsid Proteins; 0/hexon capsid protein, Adenovirus
Comments/Corrections

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