| C-reactive protein can influence the proliferation, apoptosis, and monocyte chemotactic protein-1 production of human umbilical vein endothelial cells. | |
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MedLine Citation:
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PMID: 20979529 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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C-reactive protein (CRP) has been shown to be closely associated with coronary heart disease. The serum CRP concentrations of chronic periodontitis (CP) patients were increased due to periodontal inflammation. CRP may be a potential key mediator associating CP with coronary heart disease. This study aimed to investigate the effects of CRP on human endothelial cells in vitro. CRP ranging from 0 to 10 μg/mL was adopted to imitate the chronic inflammatory conditions of periodontitis. The influences of CRP on proliferation, apoptosis, and monocyte chemotactic protein-1 (MCP-1) production of human umbilical vein endothelial cells (HUVECs) were studied through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, and enzyme-linked immunosorbent assay analysis, respectively. Compared to the blank control, 2.5 and 5.0 μg/mL CRP significantly suppressed cell proliferation by 6.9% and increased apoptosis by 10.2% and 14.6%, respectively (p < 0.05). Concentrations of 7.5 and 10.0 μg/mL CRP also induced 2.3% HUVEC proliferation suppression (p > 0.05) and significantly increased apoptosis ratio compared to that of the blank control. CRP could promote MCP-1 production of HUVECs in a concentration-dependent manner. The MCP-1 production of 10.0 μg/mL CRP group was about 15.3% higher than that of the control group. It is concluded that low concentrations of CRP, which appears in CP, inhibits cell proliferation, promotes cell apoptosis, and increases MCP-1 production in endothelium, which may initiate self-repairing function of vascular endothelium following vascular injury process. |
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Authors:
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Shujuan Guo; Shu Meng; Bin Chen; Juan Liu; Li Gao; Yafei Wu |
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Publication Detail:
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Type: Journal Article Date: 2010-10-27 |
Journal Detail:
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Title: DNA and cell biology Volume: 30 ISSN: 1557-7430 ISO Abbreviation: DNA Cell Biol. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-03-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9004522 Medline TA: DNA Cell Biol Country: United States |
Other Details:
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Languages: eng Pagination: 157-62 Citation Subset: IM |
Affiliation:
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1 State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University , Chengdu, Sichuan, China . |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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