Document Detail

Butyrate synchronization of hepatocytes: modulation of cycling and cell cycle regulated gene expression.
MedLine Citation:
PMID:  7946406     Owner:  NLM     Status:  MEDLINE    
To develop a model for studies of liver growth control, we characterized cell cycle synchronization of liver-derived cells with sodium butyrate. Exposure of cultured HTC (rat hepatoma) cells to 5 mM butyrate arrested cell growth in a reversible manner. Flow cytometric analysis revealed that butyrate-treated HTC cells were restricted in G0/G1, as well as S/G2M phases. After release from butyrate arrest, HTC cells underwent synchronous cycles of DNA synthesis and transited through S phase. Inhibition of cell growth by butyrate was associated with a complex pattern of cell cycle regulated gene expression, including a decoupling of c-fos and c-jun gene expression. Transcription of c-fos, as well as c-jun increased with butyrate arrest, whereas steady rate mRNA levels of c-jun only were increased, suggesting additional regulation of c-fos. In addition, butyrate-arrested cells exhibited a transcriptionally determined accumulation of H3 histone, C-Ha-ras and ornithine decarboxylase mRNAs, suggesting that cell cycle-related check points following the onset of S phase were modulated. An increase in c-myc mRNA levels in butyrate-arrested cells was post-transcriptionally regulated. After release from butyrate-arrest, the abundance of immediate early, as well as S phase regulated, gene expression changed coordinately with S phase cell transitions. Thus, exposure of HTC cells to butyrate modulates cell cycle regulated gene expression, inhibits cycling, and results in accumulation of cells in specific compartments. Synchronization of liver cells with butyrate should, therefore, provide a useful model for defining cell cycle-related events in response to various mitogenic stimuli.
S Gupta; G Alpini; R P Vemuru; E Hurston; D A Shafritz
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Growth factors (Chur, Switzerland)     Volume:  10     ISSN:  0897-7194     ISO Abbreviation:  Growth Factors     Publication Date:  1994  
Date Detail:
Created Date:  1994-12-08     Completed Date:  1994-12-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9000468     Medline TA:  Growth Factors     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  171-80     Citation Subset:  IM    
Marion Bessin Liver Research Center, Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461.
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MeSH Terms
Butyric Acid
Butyric Acids / pharmacology*
Cell Cycle / drug effects
Cell Division / drug effects
Cells, Cultured
Flow Cytometry
Gene Expression Regulation / drug effects*
Genes, fos / drug effects
Genes, jun / drug effects
Liver / drug effects*
Liver Neoplasms, Experimental / pathology
Grant Support
Reg. No./Substance:
0/Butyric Acids; 107-92-6/Butyric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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