| Butyrate stimulates IL-32alpha expression in human intestinal epithelial cell lines. | |
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MedLine Citation:
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PMID: 20480520 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To investigate the effects of butyrate on interleukin (IL)-32alpha expression in epithelial cell lines. METHODS: The human intestinal epithelial cell lines HT-29, SW480, and T84 were used. Intracellular IL-32alpha was determined by Western blotting analyses. IL-32alpha mRNA expression was analyzed by real-time polymerase chain reaction. RESULTS: Acetate and propionate had no effects on IL-32alpha mRNA expression. Butyrate significantly enhanced IL-32alpha expression in all cell lines. Butyrate also up-regulated IL-1beta-induced IL-32alpha mRNA expression. Butyrate did not modulate the activation of phosphatidylinositol 3-kinase (PI3K), a mediator of IL-32alpha expression. Like butyrate, trichostatin A, a histone deacetylase inhibitor, also enhanced IL-1beta-induced IL-32alpha mRNA expression. CONCLUSION: Butyrate stimulated IL-32alpha expression in epithelial cell lines. An epigenetic mechanism, such as histone hyperacetylation, might be involved in the action of butyrate on IL-32alpha expression. |
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Authors:
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Ayako Kobori; Shigeki Bamba; Hirotsugu Imaeda; Hiromitsu Ban; Tomoyuki Tsujikawa; Yasuharu Saito; Yoshihide Fujiyama; Akira Andoh |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 16 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-18 Completed Date: 2010-08-26 Revised Date: 2010-09-28 |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 2355-61 Citation Subset: IM |
Affiliation:
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Division of Gastroenterology, Department of Internal Medicine, Shiga University of Medical Science, Seta Tukinowa, Otsu, Shiga, 520-2192, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylation Blotting, Western Butyrates / pharmacology* Caco-2 Cells Dose-Response Relationship, Drug Epithelial Cells / drug effects*, immunology HT29 Cells Histone Deacetylase Inhibitors / pharmacology Histones / metabolism Humans Hydroxamic Acids / pharmacology Inflammation Mediators / metabolism* Interleukin-1beta / metabolism Interleukins / genetics, metabolism* Intestinal Mucosa / drug effects*, immunology Phosphorylation Proto-Oncogene Proteins c-akt / metabolism RNA, Messenger / metabolism Recombinant Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Butyrates; 0/Histone Deacetylase Inhibitors; 0/Histones; 0/Hydroxamic Acids; 0/IL32 protein, human; 0/Inflammation Mediators; 0/Interleukin-1beta; 0/Interleukins; 0/RNA, Messenger; 0/Recombinant Proteins; 58880-19-6/trichostatin A; EC 2.7.11.1/Proto-Oncogene Proteins c-akt |
| Comments/Corrections | |
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