| Butyrate-induced G2/M block in Caco-2 colon cancer cells is associated with decreased p34cdc2 activity. | |
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MedLine Citation:
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PMID: 10564539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Butyrate, a short-chain fatty acid, has been reported to inhibit proliferation and stimulate differentiation in multiple cancer cell lines. Whereas the effects of butyrate on cellular differentiation are well documented, the relationship between butyrate-induced differentiation and its effect on cell cycle traverse is less well understood. The purpose of this study was to investigate the effects of butyrate on the regulatory proteins of the G2/M traverse in the Caco-2 colon cancer cell model. We demonstrated that the inhibition of proliferation and increased cellular differentiation after treatment of Caco-2 cells with butyrate were associated with a significant G2/M cell cycle block. Although protein levels of the major G2/M regulatory protein, p34cdc2, were unchanged, a decrease in p34cdc2 activity was noted. Despite this decrease in activity, the inhibitory tyrosine phosphorylation of p34cdc2 was decreased, suggesting that other factors are responsible for the decreased kinase activity. The reduced activity of p34cdc2 provides a possible mechanism for the accumulation of Caco-2 cells in the G2/M cell cycle compartment following exposure to butyrate. This cell system provides a new model for studies of G2/M cell cycle perturbations. |
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Authors:
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L E Harrison; Q M Wang; G P Studzinski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.) Volume: 222 ISSN: 0037-9727 ISO Abbreviation: Proc. Soc. Exp. Biol. Med. Publication Date: 1999 Nov |
Date Detail:
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Created Date: 1999-11-30 Completed Date: 1999-11-30 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7505892 Medline TA: Proc Soc Exp Biol Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 150-6 Citation Subset: IM |
Affiliation:
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Department of Surgery, UMDNJ-New Jersey Medical School, Newark 07103, USA. Harris11@umdnj.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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metabolism Butyrates / pharmacology* CDC2 Protein Kinase / metabolism* Caco-2 Cells Cell Cycle / drug effects*, physiology Cell Differentiation / drug effects Cell Division / drug effects DNA, Neoplasm / metabolism Flow Cytometry G2 Phase Humans Kinetics Mitosis Models, Biological |
| Chemical | |
Reg. No./Substance:
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0/Butyrates; 0/DNA, Neoplasm; EC 2.7.11.22/CDC2 Protein Kinase; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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