Document Detail

Butein suppresses myofibroblastic differentiation of rat hepatic stellate cells in primary culture.
MedLine Citation:
PMID:  12724040     Owner:  NLM     Status:  MEDLINE    
Hepatic stellate cells play a key role in the pathogenesis of hepatic fibrosis. In this study, we investigate the inhibitory effect of butein on the activation and proliferation of rat primary cultured hepatic stellate cells. Possible cytotoxic effects were measured on stellate cells and hepatocytes using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The effects of butein on the production of collagen and smooth muscle alpha-actin proteins were examined at the same concentration, by western blot. The effects of butein on alpha1(I) collagen, tissue inhibitor of metalloproteinase-1, and metalloproteinase-13 gene expression in activated stellate cells were investigated by measuring mRNA levels using reverse transcription polymerase chain reaction. The effect of butein on DNA synthesis was also determined. Butein, at a concentration of 1 microg mL(-1), reduced DNA synthesis without affecting cell viability, and downregulated smooth muscle alpha-actin and type-I collagen expression, and alpha1(I) collagen and tissue inhibitor of metalloproteinase-1 mRNA expression, while treatment with butein induced metalloproteinase-13 mRNA expression. These findings suggest that butein is a potent inhibitor of stellate cell transformation.
Sun Wook Woo; Sung Hee Lee; Hee-Chul Kang; Eun-Jeon Park; Yu-Zhe Zhao; Youn-Chul Kim; Dong Hwan Sohn
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pharmacy and pharmacology     Volume:  55     ISSN:  0022-3573     ISO Abbreviation:  J. Pharm. Pharmacol.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-05-01     Completed Date:  2003-07-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376363     Medline TA:  J Pharm Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  347-52     Citation Subset:  IM    
Department of Pharmacy, Medicinal Resources Research Center, Wonkwang University, Iksan, Jeonbuk 570-749, South Korea.
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MeSH Terms
Actins / biosynthesis
Antimetabolites / diagnostic use
Blotting, Western
Bromodeoxyuridine / diagnostic use
Cell Differentiation / drug effects
Cell Survival / drug effects
Cells, Cultured
Chalcone / analogs & derivatives*,  pharmacology*
Collagen / biosynthesis
Collagenases / biosynthesis
DNA / biosynthesis
Fibroblasts / drug effects
Liver / cytology*,  drug effects
Matrix Metalloproteinase 13
Phosphodiesterase Inhibitors / pharmacology*
Platelet-Derived Growth Factor / pharmacology
RNA, Messenger / biosynthesis
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Tissue Inhibitor of Metalloproteinase-1 / biosynthesis
Reg. No./Substance:
0/Actins; 0/Antimetabolites; 0/Chalcones; 0/Phosphodiesterase Inhibitors; 0/Platelet-Derived Growth Factor; 0/RNA, Messenger; 0/Tissue Inhibitor of Metalloproteinase-1; 487-52-5/butein; 59-14-3/Bromodeoxyuridine; 9007-34-5/Collagen; 9007-49-2/DNA; 94-41-7/Chalcone; EC 3.4.24.-/Collagenases; EC 3.4.24.-/Matrix Metalloproteinase 13; EC 3.4.24.-/Mmp13 protein, rat

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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