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Butanol production from acid hydrolyzed corn fiber with Clostridium beijerinckii mutant.
MedLine Citation:
PMID:  23305897     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Sulfuric acid treated corn fiber hydrolysate (SACFH) inhibited cell growth and the production of butanol (4.7±0.2g/L) by Clostridium beijerinckii IB4 in P2 medium. Optimal medium components were determined using fractional factorial design. NH(4)HCO(3), FeSO(4)·7H(2)O and CaCO(3) were demonstrated to be significant components in the production of butanol. The Box-Behnken design and a corresponding quadratic model were used to predict medium components (NH(4)HCO(3) 1.96g/L, FeSO(4)·7H(2)O 0.26g/L and CaCO(3) 3.15g/L) and butanol yield (9.5g/L). The confirmation experiment, under the predicted optimal conditions, yielded a butanol level of 9.5±0.1g/L. This study indicates that the Box-Behnken design is an effective approach for screening the optimal medium components required for the production of butanol. It also demonstrates that SACFH, which has high levels of inhibitors such as furan and phenolic compounds, may be used as a renewable carbon source in the production of biofuels.
Authors:
Teng-Fei Du; Ai-Yong He; Hao Wu; Jia-Nan Chen; Xiang-Ping Kong; Jun-Li Liu; Min Jiang; Ping-Kai Ouyang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-15
Journal Detail:
Title:  Bioresource technology     Volume:  -     ISSN:  1873-2976     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2013-1-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211816, People's Republic of China.
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