| Burn injury reduces neutrophil directional migration speed in microfluidic devices. | |
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MedLine Citation:
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PMID: 20689600 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Thermal injury triggers a fulminant inflammatory cascade that heralds shock, end-organ failure, and ultimately sepsis and death. Emerging evidence points to a critical role for the innate immune system, and several studies had documented concurrent impairment in neutrophil chemotaxis with these post-burn inflammatory changes. While a few studies suggest that a link between neutrophil motility and patient mortality might exist, so far, cumbersome assays have prohibited exploration of the prognostic and diagnostic significance of chemotaxis after burn injury. To address this need, we developed a microfluidic device that is simple to operate and allows for precise and robust measurements of chemotaxis speed and persistence characteristics at single-cell resolution. Using this assay, we established a reference set of migration speed values for neutrophils from healthy subjects. Comparisons with samples from burn patients revealed impaired directional migration speed starting as early as 24 hours after burn injury, reaching a minimum at 72-120 hours, correlated to the size of the burn injury and potentially serving as an early indicator for concurrent infections. Further characterization of neutrophil chemotaxis using this new assay may have important diagnostic implications not only for burn patients but also for patients afflicted by other diseases that compromise neutrophil functions. |
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Authors:
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Kathryn L Butler; Vijayakrishnan Ambravaneswaran; Nitin Agrawal; Maryelizabeth Bilodeau; Mehmet Toner; Ronald G Tompkins; Shawn Fagan; Daniel Irimia |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-07-30 |
Journal Detail:
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Title: PloS one Volume: 5 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2010 |
Date Detail:
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Created Date: 2010-08-06 Completed Date: 2010-10-28 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e11921 Citation Subset: IM |
Affiliation:
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Surgery Department, Massachusetts General Hospital, Shriners Hospital for Children, and Harvard Medical School, Boston, Massachusetts, United States of America. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Burns / immunology*, pathology* Cell Movement / physiology* Female Humans Male Microfluidic Analytical Techniques / methods* Middle Aged Neutrophils / cytology* Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM092804-01/GM/NIGMS NIH HHS; R01 GM092804-02/GM/NIGMS NIH HHS; R01GM092804/GM/NIGMS NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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