Document Detail


Burn injury reduces neutrophil directional migration speed in microfluidic devices.
MedLine Citation:
PMID:  20689600     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thermal injury triggers a fulminant inflammatory cascade that heralds shock, end-organ failure, and ultimately sepsis and death. Emerging evidence points to a critical role for the innate immune system, and several studies had documented concurrent impairment in neutrophil chemotaxis with these post-burn inflammatory changes. While a few studies suggest that a link between neutrophil motility and patient mortality might exist, so far, cumbersome assays have prohibited exploration of the prognostic and diagnostic significance of chemotaxis after burn injury. To address this need, we developed a microfluidic device that is simple to operate and allows for precise and robust measurements of chemotaxis speed and persistence characteristics at single-cell resolution. Using this assay, we established a reference set of migration speed values for neutrophils from healthy subjects. Comparisons with samples from burn patients revealed impaired directional migration speed starting as early as 24 hours after burn injury, reaching a minimum at 72-120 hours, correlated to the size of the burn injury and potentially serving as an early indicator for concurrent infections. Further characterization of neutrophil chemotaxis using this new assay may have important diagnostic implications not only for burn patients but also for patients afflicted by other diseases that compromise neutrophil functions.
Authors:
Kathryn L Butler; Vijayakrishnan Ambravaneswaran; Nitin Agrawal; Maryelizabeth Bilodeau; Mehmet Toner; Ronald G Tompkins; Shawn Fagan; Daniel Irimia
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-07-30
Journal Detail:
Title:  PloS one     Volume:  5     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2010  
Date Detail:
Created Date:  2010-08-06     Completed Date:  2010-10-28     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e11921     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Burns / immunology*,  pathology*
Cell Movement / physiology*
Female
Humans
Male
Microfluidic Analytical Techniques / methods*
Middle Aged
Neutrophils / cytology*
Young Adult
Grant Support
ID/Acronym/Agency:
P50 GM021700/GM/NIGMS NIH HHS; R01 GM092804/GM/NIGMS NIH HHS; R01 GM092804-02/GM/NIGMS NIH HHS; R01GM092804/GM/NIGMS NIH HHS
Comments/Corrections

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