Document Detail


Burkholderia cenocepacia phenotypic clonal variation during a 3.5-year colonization in the lungs of a cystic fibrosis patient.
MedLine Citation:
PMID:  21536796     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chronic lung infection is the major cause of morbidity and premature mortality in cystic fibrosis (CF) patients. Bacteria of the Burkholderia cepacia complex are the most threatening pathogens in CF, and a better understanding of how these bacteria adapt to the CF airway environment and resist the host defense mechanisms and therapeutically administered antibiotics is crucial. To provide clues to the adaptive strategies adopted by Burkholderia cenocepacia during long-term colonization, we carried out a phenotypic assessment of 11 clonal variants obtained at the major Portuguese CF Center in Lisbon from sputa of the same CF patient during 3.5 years of colonization of the lungs, until the patient's death with cepacia syndrome. Phenotypic characterization included susceptibility assays against different classes of antimicrobials and characterization of cell motility, cell hydrophobicity and zeta potential, colony and cell morphology, fatty acid composition, growth under iron limitation/load conditions, exopolysaccharide production, and size of the biofilms formed. The results suggest the occurrence of clonal expansion during long-term colonization. For a number of the characteristics tested, no isolation time-dependent consistent alteration pattern could be identified. However, the values for antimicrobial susceptibility and swarming motility for the first B. cenocepacia isolate, thought to have initiated the infection, were consistently above those for the clonal variants obtained during the course of infection, and the opposite was found for the zeta potential. The adaptive strategy for long-term colonization, described here for the first time, involved the alteration of membrane fatty acid composition, in particular a reduction of the degree of fatty acid saturation, in the B. cenocepacia variants retrieved, along with the deterioration of pulmonary function and severe oxygen limitation.
Authors:
Carla P Coutinho; Carla C C R de Carvalho; Andreia Madeira; Ana Pinto-de-Oliveira; Isabel Sá-Correia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-05-02
Journal Detail:
Title:  Infection and immunity     Volume:  79     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-21     Completed Date:  2011-09-02     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2950-60     Citation Subset:  IM    
Affiliation:
Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal.
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / pharmacology
Base Sequence
Burkholderia Infections / microbiology*,  physiopathology
Burkholderia cenocepacia* / drug effects,  genetics,  isolation & purification,  physiology
Cell Membrane / chemistry
Cystic Fibrosis / microbiology*,  physiopathology
Fatty Acids / analysis
Humans
Lung / microbiology*
Lung Diseases / microbiology
Membrane Lipids / analysis
Microbial Sensitivity Tests
Molecular Sequence Data
Phenotype
Polymorphism, Genetic
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Fatty Acids; 0/Membrane Lipids
Comments/Corrections

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