| Burkholderia cenocepacia disrupts host cell actin cytoskeleton by inactivating Rac and Cdc42. | |
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MedLine Citation:
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PMID: 22023324 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Burkholderia cenocepacia, a member of the Burkholderia cepacia complex, is an opportunistic pathogen that causes devastating infections in patients with cystic fibrosis. The ability of B. cenocepacia to survive within host cells could contribute significantly to its virulence in immunocompromised patients. In this study, we explored the mechanisms that enable B. cenocepacia to survive inside macrophages. We found that B. cenocepacia disrupts the actin cytoskeleton of infected macrophages, drastically altering their morphology. Submembranous actin undergoes depolymerization, leading to cell retraction. The bacteria perturb actin architecture by inactivating Rho-family GTPases, particularly Rac1 and Cdc42. GTPase inactivation follows internalization of viable B. cenocepacia and compromises phagocyte function: macropinocytosis and phagocytosis are markedly inhibited, likely impairing the microbicidal and antigen-presenting capability of infected macrophages. The type VI secretion system is essential for the bacteria to elicit these changes. This is the first report demonstrating inactivation of Rho-family GTPases by a member of the B. cepacia complex. |
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Authors:
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Ronald S Flannagan; Valentin Jaumouillé; Kassidy K Huynh; Jonathan D Plumb; Gregory P Downey; Miguel A Valvano; Sergio Grinstein |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-25 |
Journal Detail:
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Title: Cellular microbiology Volume: - ISSN: 1462-5822 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883691 Medline TA: Cell Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 Blackwell Publishing Ltd. |
Affiliation:
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Program in Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8 Departments of Medicine and Pediatrics, National Jewish Health, Denver, CO, and Departments of Medicine and Integrated Department of Immunology, University of Colorado, Aurora, CO, USA Centre for Human Immunology and the Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada, N6A 5C1. |
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