Document Detail

The Burkholderia cenocepacia BDSF quorum sensing fatty acid is synthesized by a bifunctional crotonase homologue having both dehydratase and thioesterase activities.
MedLine Citation:
PMID:  22221091     Owner:  NLM     Status:  MEDLINE    
Signal molecules of the diffusible signal factor (DSF) family have been shown recently to be involved in regulation of pathogenesis and biofilm formation in diverse Gram-negative bacteria. DSF signals are reported to be active not only on their cognate bacteria, but also on unrelated bacteria and the pathogenic yeast, Candida albicans. DSFs are monounsaturated fatty acids of medium chain length containing an unusual cis-2 double bond. Although genetic analyses had identified genes involved in DSF synthesis, the pathway of DSF synthesis was unknown. The DSF of the important human pathogen Burkholderia cenocepacia (called BDSF) is cis-2-dodecenoic acid. We report that BDSF is synthesized from a fatty acid synthetic intermediate, the acyl carrier protein (ACP) thioester of 3-hydroxydodecanoic acid. This intermediate is intercepted by protein Bcam0581 and converted to cis-2-dodecenoyl-ACP. Bcam0581 is annotated as a homologue of crotonase, the first enzyme of the fatty acid degradation pathway. We demonstrated Bcam0581to be a bifunctional protein that not only catalysed dehydration of 3-hydroxydodecanoyl-ACP to cis-2-dodecenoyl-ACP, but also cleaved the thioester bond to give the free acid. Both activities required the same set of active-site residues. Although dehydratase and thioesterase activities are known activities of the crotonase superfamily, Bcam0581 is the first protein shown to have both activities.
Hongkai Bi; Quin H Christensen; Youjun Feng; Haihong Wang; John E Cronan
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-01-29
Journal Detail:
Title:  Molecular microbiology     Volume:  83     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-03     Completed Date:  2012-05-29     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  840-55     Citation Subset:  IM    
Copyright Information:
© 2012 Blackwell Publishing Ltd.
Departments of Microbiology Biochemistry, B103 Chemical and Life Sciences Laboratory, University of Illinois, 601 S. Goodwin Ave, Urbana, IL 61801, USA.
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MeSH Terms
Acyl Carrier Protein / metabolism
Amino Acid Sequence
Burkholderia cenocepacia / metabolism*,  physiology
Enoyl-CoA Hydratase / metabolism*
Fatty Acids, Monounsaturated / metabolism*
Hydro-Lyases / metabolism*
Lauric Acids / metabolism
Models, Biological
Models, Chemical
Molecular Sequence Data
Palmitoyl-CoA Hydrolase / metabolism*
Quorum Sensing
Sequence Homology, Amino Acid
Grant Support
AI15650/AI/NIAID NIH HHS; R01 AI015650-34/AI/NIAID NIH HHS; R01 AI015650-35/AI/NIAID NIH HHS; R01 AI015650-36/AI/NIAID NIH HHS
Reg. No./Substance:
0/2-dodecenoic acid; 0/Acyl Carrier Protein; 0/Fatty Acids, Monounsaturated; 0/Lauric Acids; 1883-13-2/3-hydroxydodecanoic acid; EC Hydrolase; EC 4.2.1.-/Hydro-Lyases; EC Hydratase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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