Document Detail


Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions.
MedLine Citation:
PMID:  20865170     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacteria that live in the environment have evolved pathways specialized to defend against eukaryotic organisms or other bacteria. In this manuscript, we systematically examined the role of the five type VI secretion systems (T6SSs) of Burkholderia thailandensis (B. thai) in eukaryotic and bacterial cell interactions. Consistent with phylogenetic analyses comparing the distribution of the B. thai T6SSs with well-characterized bacterial and eukaryotic cell-targeting T6SSs, we found that T6SS-5 plays a critical role in the virulence of the organism in a murine melioidosis model, while a strain lacking the other four T6SSs remained as virulent as the wild-type. The function of T6SS-5 appeared to be specialized to the host and not related to an in vivo growth defect, as ΔT6SS-5 was fully virulent in mice lacking MyD88. Next we probed the role of the five systems in interbacterial interactions. From a group of 31 diverse bacteria, we identified several organisms that competed less effectively against wild-type B. thai than a strain lacking T6SS-1 function. Inactivation of T6SS-1 renders B. thai greatly more susceptible to cell contact-induced stasis by Pseudomonas putida, Pseudomonas fluorescens and Serratia proteamaculans-leaving it 100- to 1000-fold less fit than the wild-type in competition experiments with these organisms. Flow cell biofilm assays showed that T6S-dependent interbacterial interactions are likely relevant in the environment. B. thai cells lacking T6SS-1 were rapidly displaced in mixed biofilms with P. putida, whereas wild-type cells persisted and overran the competitor. Our data show that T6SSs within a single organism can have distinct functions in eukaryotic versus bacterial cell interactions. These systems are likely to be a decisive factor in the survival of bacterial cells of one species in intimate association with those of another, such as in polymicrobial communities present both in the environment and in many infections.
Authors:
Sandra Schwarz; T Eoin West; Frédéric Boyer; Wen-Chi Chiang; Mike A Carl; Rachel D Hood; Laurence Rohmer; Tim Tolker-Nielsen; Shawn J Skerrett; Joseph D Mougous
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-08-26
Journal Detail:
Title:  PLoS pathogens     Volume:  6     ISSN:  1553-7374     ISO Abbreviation:  PLoS Pathog.     Publication Date:  2010  
Date Detail:
Created Date:  2010-09-24     Completed Date:  2010-12-02     Revised Date:  2014-09-05    
Medline Journal Info:
Nlm Unique ID:  101238921     Medline TA:  PLoS Pathog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e1001068     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteria / genetics,  growth & development,  metabolism
Bacterial Proteins / genetics,  immunology*
Bacterial Secretion Systems / genetics,  immunology*
Biofilms / growth & development
Burkholderia / genetics,  immunology*,  pathogenicity*
Burkholderia Infections / genetics,  immunology
Host-Parasite Interactions / genetics,  immunology*
Mice
Phylogeny
Virulence / genetics,  immunology
Grant Support
ID/Acronym/Agency:
AI057141/AI/NIAID NIH HHS; AI080609/AI/NIAID NIH HHS; K08 HL094759/HL/NHLBI NIH HHS; K08 HL094759-02/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins
Comments/Corrections

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