Document Detail

Bulk staining of smears: no demonstrated risk of bacilli transfer from a positive to a negative smear.
MedLine Citation:
PMID:  18492337     Owner:  NLM     Status:  MEDLINE    
Despite a theoretical risk of transfer of bacilli from a positive to a negative smear, bulk staining is routinely performed in many laboratories. To assess this risk in our laboratory, two smears were made from each sputum specimen and stained with auramine: one smear was stained on a rack and the second using the bulk method. Smears were read blind using a fluorescence microscope. A total of 811 sputum specimens were analysed. No acid-fast bacilli transfer was observed even when staining solution jars had not been renewed for 3 days. Bulk staining is rapid and cheap, and could be used in laboratories with a high workload in low-resource settings.
D Affolabi; M Odoun; C N Sanoussi; B Tanimomo-Kledjo; D Saïzonou; K Soumaïla; L Kestens; S Y Anagonou; F Portaels
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease     Volume:  12     ISSN:  1027-3719     ISO Abbreviation:  Int. J. Tuberc. Lung Dis.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-21     Completed Date:  2008-09-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706389     Medline TA:  Int J Tuberc Lung Dis     Country:  France    
Other Details:
Languages:  eng     Pagination:  683-5     Citation Subset:  IM    
Laboratoire de Référence des Mycobactéries, Cotonou, Bénin.
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MeSH Terms
Bacteriological Techniques*
Microscopy, Fluorescence*
Sputum / microbiology*
Staining and Labeling
Tuberculosis, Pulmonary / diagnosis

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