Document Detail


Bulk staining of smears: no demonstrated risk of bacilli transfer from a positive to a negative smear.
MedLine Citation:
PMID:  18492337     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Despite a theoretical risk of transfer of bacilli from a positive to a negative smear, bulk staining is routinely performed in many laboratories. To assess this risk in our laboratory, two smears were made from each sputum specimen and stained with auramine: one smear was stained on a rack and the second using the bulk method. Smears were read blind using a fluorescence microscope. A total of 811 sputum specimens were analysed. No acid-fast bacilli transfer was observed even when staining solution jars had not been renewed for 3 days. Bulk staining is rapid and cheap, and could be used in laboratories with a high workload in low-resource settings.
Authors:
D Affolabi; M Odoun; C N Sanoussi; B Tanimomo-Kledjo; D Saïzonou; K Soumaïla; L Kestens; S Y Anagonou; F Portaels
Related Documents :
12470347 - The use of preimplantation genetic diagnosis in sex selection for family balancing in i...
1894927 - Selective pregnancy reduction: medical attitudes, legal implications, and a viable alte...
2245847 - Establishment of pregnancies in humans after transcervical transfer of gametes immediat...
17482177 - Management of poor responders: can outcomes be improved with a novel gonadotropin-relea...
18450657 - Duration of antidepressant use during pregnancy and risk of major congenital malformati...
2249627 - A simple and sensitive microtiter plate estrogen bioassay based on stimulation of alkal...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease     Volume:  12     ISSN:  1027-3719     ISO Abbreviation:  Int. J. Tuberc. Lung Dis.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-21     Completed Date:  2008-09-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706389     Medline TA:  Int J Tuberc Lung Dis     Country:  France    
Other Details:
Languages:  eng     Pagination:  683-5     Citation Subset:  IM    
Affiliation:
Laboratoire de Référence des Mycobactéries, Cotonou, Bénin. affolabi_dissou@yahoo.fr
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Bacteriological Techniques*
Humans
Microscopy, Fluorescence*
Sputum / microbiology*
Staining and Labeling
Tuberculosis, Pulmonary / diagnosis

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  General practitioners' knowledge of childhood asthma in Delhi, India.
Next Document:  High tuberculosis prevalence in a psychiatric hospital in Vietnam.