| Bulk shear viscosities of endogenous and exogenous lung surfactants. | |
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MedLine Citation:
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PMID: 11792632 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bulk shear viscosities were measured with a cone and plate microviscometer as a function of concentration, shear rate, and temperature for lavaged calf lung surfactant (LS), Exosurf, Infasurf, Survanta, and synthetic lipid mixtures dispersed in normal saline. Viscosity increased with phospholipid concentration for all surfactants, but its magnitude and shear dependence varied widely among the different preparations. Saline dispersions of Exosurf and synthetic phospholipids had low viscosities of only a few centipoise (cp) and exhibited minimal shear dependence. LS, Infasurf, Survanta, and lipid mixtures containing palmitic acid and tripalmitin had larger non-Newtonian viscosities that increased as shear rate decreased. At 35 mg of phospholipid/ml and 37 degrees C, viscosity values were 52.3 cp (Survanta), 31.1 cp (LS), and 25 cp (Infasurf) at a shear rate of 77 s(-1) and 16.9 cp (Survanta), 10.1 cp (LS), and 6.6 cp (Infasurf) at 770 s(-1). At 25 mg of phospholipid/ml and 37 degrees C, viscosity values at 77 s(-1) were 28.8 cp (Survanta), 4.7 cp (LS), and 12.5 cp (Infasurf). At fixed shear rate, viscosity was substantially decreased at 23 degrees C compared with 37 degrees C for LS and Infasurf but was increased for Survanta. Calcium (5 mM) greatly reduced the viscosity of both Survanta and Infasurf at 37 degrees C. Studies on synthetic mixtures indicated that phospholipid/apoprotein interactions were important in the rheology of lung-derived surfactants and that palmitic acid and tripalmitin contributed to the increased viscosity of Survanta. The viscous behavior of clinical exogenous surfactants potentially influences their delivery and distribution in lungs and varies significantly with composition, concentration, temperature, ionic environment, and physical formulation. |
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Authors:
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David M King; Zhengdong Wang; Harvey J Palmer; Bruce A Holm; Robert H Notter |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of physiology. Lung cellular and molecular physiology Volume: 282 ISSN: 1040-0605 ISO Abbreviation: Am. J. Physiol. Lung Cell Mol. Physiol. Publication Date: 2002 Feb |
Date Detail:
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Created Date: 2002-01-16 Completed Date: 2002-03-07 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 100901229 Medline TA: Am J Physiol Lung Cell Mol Physiol Country: United States |
Other Details:
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Languages: eng Pagination: L277-84 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, University of Rochester, Rochester, New York 14642, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Products* Calcium / chemistry Cattle Drug Combinations Fatty Alcohols / chemistry Phosphorylcholine* Polyethylene Glycols / chemistry Pulmonary Surfactants / chemistry* Sodium Chloride Stress, Mechanical Temperature Viscosity |
| Grant Support | |
ID/Acronym/Agency:
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HL-56176/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Products; 0/Drug Combinations; 0/Fatty Alcohols; 0/Polyethylene Glycols; 0/Pulmonary Surfactants; 0/calfactant; 107-73-3/Phosphorylcholine; 108778-82-1/beractant; 7440-70-2/Calcium; 7647-14-5/Sodium Chloride; 99732-49-7/Exosurf |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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