Document Detail

Buffer modulation of menadione-induced oxidative stress in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  19843376     Owner:  NLM     Status:  MEDLINE    
The objective of this study was to compare, in vivo, the effects of bicarbonate and phosphate buffers on survival and menadione-induced oxidative stress in yeast cells. The latter were treated with different concentrations of menadione in the presence of these two buffers. At 25 mM concentration of buffers, menadione only slightly reduced yeast surviving; at 50 mM concentration, cell killing by menadione was much more pronounced in bicarbonate than in phosphate buffer. Although the content of protein carbonyl groups did not show development of oxidative stress under menadione-induced stress, inactivation of aconitase and decrease in glutathione level mirrored its induction. However, cellular glutathione and aconitase activity decrease did not correlate with yeast survival. In vitro, aconitase was more quickly inactivated in 50 mM carbonate, than in 50 mM phosphate buffer. The possible involvement of the carbonate radical in these processes is discussed.
Oleh V Lushchak; Maria M Bayliak; Olha V Korobova; Rodney L Levine; Volodymyr I Lushchak
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Redox report : communications in free radical research     Volume:  14     ISSN:  1743-2928     ISO Abbreviation:  Redox Rep.     Publication Date:  2009  
Date Detail:
Created Date:  2009-10-21     Completed Date:  2010-01-15     Revised Date:  2013-03-21    
Medline Journal Info:
Nlm Unique ID:  9511366     Medline TA:  Redox Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  214-20     Citation Subset:  IM    
Department of Biochemistry, Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine.
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MeSH Terms
Aconitate Hydratase / metabolism
Antifibrinolytic Agents / pharmacology*
Antioxidants / metabolism
Bicarbonates / pharmacology*
Enzyme Activation / drug effects
Glutathione / metabolism
Oxidative Stress / drug effects*
Phosphates / pharmacology*
Saccharomyces cerevisiae / drug effects*,  metabolism
Vitamin K 3 / pharmacology*
Grant Support
Reg. No./Substance:
0/Antifibrinolytic Agents; 0/Antioxidants; 0/Bicarbonates; 0/Phosphates; 58-27-5/Vitamin K 3; 70-18-8/Glutathione; EC Hydratase

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