| Buffalo (Bubalus bubalis) interleukin-12: analysis of expression profiles and functional cross-reactivity with bovine system. | |
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MedLine Citation:
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PMID: 16112195 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Interleukin-12, a heterodimeric pro-inflammatory cytokine, from water buffaloes (Bubalus bubalis) was analyzed for its for its tissue specific expression and functionality. Concanavalin A stimulated splenocytes displayed an up-regulation of the IL-12 p40 subunit 8-24h post-stimulation, whereas the p35 subunit did not show any quantitative variation at different time intervals. Basal level expressions of both the subunits were observed by RT-PCR in spleen. In addition p40 transcripts could be detected in liver and p35 in brain and muscle tissues as well in very low levels. Functional recombinant buffalo IL-12 was expressed in HEK 293T cells as a heterodimer using foot-and-mouth disease virus 2A polypeptide as a linker. Culture supernatants from transfected cells contained a hetero-dimeric p70 subunit as revealed in western blot of the proteins separated by native polyacrylamide gel electrophoresis (PAGE) using a monoclonal antibody against bovine IL-12 p40. IL-12 containing culture supernatant induced production of nitric oxide in cultured splenocytes of both buffalo and bovine origin. Our study reveals that buffalo IL-12, which shares a high-level sequence identity with bovine IL-12, also has functional cross-reactivity with the bovine immune cells. |
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Authors:
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Avinash Premraj; E Sreekumar; Mamta Jain; T J Rasool |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-08-19 |
Journal Detail:
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Title: Molecular immunology Volume: 43 ISSN: 0161-5890 ISO Abbreviation: Mol. Immunol. Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2005-12-20 Completed Date: 2006-03-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7905289 Medline TA: Mol Immunol Country: England |
Other Details:
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Languages: eng Pagination: 822-9 Citation Subset: IM |
Affiliation:
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Animal Biotechnology Laboratory, Rajiv Gandhi Center for Biotechnology (RGCB), Jagathy, Thycaud PO, Trivandrum 695014, Kerala, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Buffaloes / immunology* Cattle Cells, Cultured Concanavalin A / pharmacology Dimerization Gene Expression Profiling Genetic Vectors / genetics Humans Interleukin-12 / biosynthesis*, genetics, pharmacology Interleukin-12 Subunit p40 Leukocytes, Mononuclear / drug effects, metabolism Nitric Oxide / biosynthesis Protein Subunits / biosynthesis*, genetics, pharmacology RNA, Messenger / biosynthesis Recombinant Proteins / biosynthesis, genetics, pharmacology Spleen / cytology, drug effects, metabolism Tissue Distribution Transcription, Genetic Transgenes Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-12 Subunit p40; 0/Protein Subunits; 0/RNA, Messenger; 0/Recombinant Proteins; 10102-43-9/Nitric Oxide; 11028-71-0/Concanavalin A; 187348-17-0/Interleukin-12 |
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