Document Detail


Buffalo (Bubalus bubalis) interleukin-12: analysis of expression profiles and functional cross-reactivity with bovine system.
MedLine Citation:
PMID:  16112195     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interleukin-12, a heterodimeric pro-inflammatory cytokine, from water buffaloes (Bubalus bubalis) was analyzed for its for its tissue specific expression and functionality. Concanavalin A stimulated splenocytes displayed an up-regulation of the IL-12 p40 subunit 8-24h post-stimulation, whereas the p35 subunit did not show any quantitative variation at different time intervals. Basal level expressions of both the subunits were observed by RT-PCR in spleen. In addition p40 transcripts could be detected in liver and p35 in brain and muscle tissues as well in very low levels. Functional recombinant buffalo IL-12 was expressed in HEK 293T cells as a heterodimer using foot-and-mouth disease virus 2A polypeptide as a linker. Culture supernatants from transfected cells contained a hetero-dimeric p70 subunit as revealed in western blot of the proteins separated by native polyacrylamide gel electrophoresis (PAGE) using a monoclonal antibody against bovine IL-12 p40. IL-12 containing culture supernatant induced production of nitric oxide in cultured splenocytes of both buffalo and bovine origin. Our study reveals that buffalo IL-12, which shares a high-level sequence identity with bovine IL-12, also has functional cross-reactivity with the bovine immune cells.
Authors:
Avinash Premraj; E Sreekumar; Mamta Jain; T J Rasool
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-08-19
Journal Detail:
Title:  Molecular immunology     Volume:  43     ISSN:  0161-5890     ISO Abbreviation:  Mol. Immunol.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2005-12-20     Completed Date:  2006-03-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905289     Medline TA:  Mol Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  822-9     Citation Subset:  IM    
Affiliation:
Animal Biotechnology Laboratory, Rajiv Gandhi Center for Biotechnology (RGCB), Jagathy, Thycaud PO, Trivandrum 695014, Kerala, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Buffaloes / immunology*
Cattle
Cells, Cultured
Concanavalin A / pharmacology
Dimerization
Gene Expression Profiling
Genetic Vectors / genetics
Humans
Interleukin-12 / biosynthesis*,  genetics,  pharmacology
Interleukin-12 Subunit p40
Leukocytes, Mononuclear / drug effects,  metabolism
Nitric Oxide / biosynthesis
Protein Subunits / biosynthesis*,  genetics,  pharmacology
RNA, Messenger / biosynthesis
Recombinant Proteins / biosynthesis,  genetics,  pharmacology
Spleen / cytology,  drug effects,  metabolism
Tissue Distribution
Transcription, Genetic
Transgenes
Up-Regulation
Chemical
Reg. No./Substance:
0/Interleukin-12 Subunit p40; 0/Protein Subunits; 0/RNA, Messenger; 0/Recombinant Proteins; 10102-43-9/Nitric Oxide; 11028-71-0/Concanavalin A; 187348-17-0/Interleukin-12

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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