| Bubble-induced platelet aggregation in a rat model of decompression sickness. | |
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MedLine Citation:
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PMID: 19850726 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies have highlighted that bubble-induced platelet aggregation is a predictor index of decompression sickness (DCS) severity in animals and bubble formation after a single air dive in humans. The present study attempted to investigate plasmatic indexes of the coagulation system and platelet activation in our rat model of DCS. Male Sprague-Dawley rats were assigned to one experimental group with a hyperbaric exposure and one control group maintained at atmospheric pressure. Rats were compressed to 1,000 kPa (90 m saltwater) for 45 min while breathing air. The onset of death time and DCS symptoms were recorded during a 30-min observed period after rats had surfaced. Plasmatic indexes were platelet factor 4 (PF4) for platelet activation, soluble glycoprotein V (sGPV) for thrombin generation, and thrombin-antithrombin complexes for the coagulation system. Blood samples for a platelet count and markers were taken 3 wk before the experimental protocol and within the 30 min after rats had surfaced. We confirmed a correlation between the percent fall in platelet count and DCS severity. Plasmatic levels of sGPV and PF4 were significantly increased after the hyperbaric exposure, with no change in the control group. The present study confirms platelet consumption as a potential index for evaluating decompression stress and DCS severity. The results point to the participation of thrombin generation in the coagulation cascade and platelet activation in bubble-induced platelet aggregation. In our animal model of DCS, the results cannot prejudge the mechanisms of platelet activation between bubble-induced vessel wall injury and bubble-blood component interactions. |
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Authors:
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Jean-Michel Pontier; Nicolas Vall??e; Lionel Bourdon |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-22 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 107 ISSN: 1522-1601 ISO Abbreviation: J. Appl. Physiol. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-12-02 Completed Date: 2010-02-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 1825-9 Citation Subset: IM |
Affiliation:
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1Medicine Department, French Navy Diving School, Toulon Army, France. jm.pontier@free.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Air Pressure* Animals Antithrombin III Blood Coagulation Decompression Sickness / metabolism* Disease Models, Animal Embolism, Air / blood* Male Peptide Hydrolases / blood Platelet Aggregation* Platelet Count Platelet Factor 4 / blood Platelet Glycoprotein GPIb-IX Complex / metabolism Random Allocation Rats Rats, Sprague-Dawley Regression Analysis Severity of Illness Index Statistics, Nonparametric |
| Chemical | |
Reg. No./Substance:
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0/Platelet Glycoprotein GPIb-IX Complex; 0/antithrombin III-protease complex; 37270-94-3/Platelet Factor 4; 9000-94-6/Antithrombin III; EC 3.4.-/Peptide Hydrolases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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