| Btn2p is involved in ethanol tolerance and biofilm formation in flor yeast. | |
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MedLine Citation:
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PMID: 18554307 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Flor yeasts are a particular kind of Saccharomyces cerevisiae strains involved in Sherry wine biological ageing. During this process, yeasts form a film on the wine surface and use ethanol as a carbon source, producing acetaldehyde as a by-product. Acetaldehyde induces BTN2 transcription in laboratory strains. Btn2p is involved in the control of the subcellular localization of different proteins. The BTN2 gene shows a complex expression pattern in wine yeast, increasing its expression by acetaldehyde, but repressing it by ethanol. A flor yeast strain transcribes more BTN2 than a first fermentation yeast during growth, but less under different stress conditions. BTN2 deletion decreases flor yeast resistance to high ethanol concentrations. Surprisingly, this effect is suppressed by the addition of high amounts of amino acids to the growth medium, indicating that the role of Btn2p protein in amino acid transport is important for ethanol resistance. Btn2p deletion increases the fermentative capacity of flor yeast and its overexpression prevents its growth on nonfermentable carbon sources. BTN2 deletion also affects the biofilm formation ability of flor yeast, and it increases its sliding motility, resulting in increased mat formation. This correlates with an increased transcription of the FLO11 gene, a gene essential for biofilm formation. |
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Authors:
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Marisa Espinazo-Romeu; Jesús M Cantoral; Emilia Matallana; Agustín Aranda |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-06-12 |
Journal Detail:
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Title: FEMS yeast research Volume: 8 ISSN: 1567-1356 ISO Abbreviation: FEMS Yeast Res. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-12-04 Completed Date: 2009-01-16 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 101085384 Medline TA: FEMS Yeast Res Country: England |
Other Details:
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Languages: eng Pagination: 1127-36 Citation Subset: IM |
Affiliation:
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Department of Biotechnology, IATA (CSIC), Paterna, Valencia, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetaldehyde
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metabolism,
pharmacology Amino Acid Transport Systems / metabolism* Biofilms / growth & development* Biotechnology Culture Media Ethanol / metabolism, pharmacology* Gene Deletion Gene Expression Regulation, Fungal* Heat-Shock Response Membrane Glycoproteins Membrane Proteins / genetics, metabolism Saccharomyces cerevisiae / drug effects*, growth & development*, metabolism, physiology Saccharomyces cerevisiae Proteins / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acid Transport Systems; 0/BTN2 protein, S cerevisiae; 0/Culture Media; 0/MUC1 protein, S cerevisiae; 0/Membrane Glycoproteins; 0/Membrane Proteins; 0/Saccharomyces cerevisiae Proteins; 64-17-5/Ethanol; 75-07-0/Acetaldehyde |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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