Document Detail


Bryostatin 1-activated T cells can traffic and mediate tumor regression.
MedLine Citation:
PMID:  1528028     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adoptive immunotherapy in humans may be limited by the lack of autologous tumor cells to activate and expand tumor-specific T cells. Pharmacologic manipulation of protein kinase C (PKC) and intracellular calcium may substitute for tumor antigen and stimulate T cells for adoptive immunotherapy. In the present study, we evaluated the ability of the PKC activator Bryostatin 1 (B) plus the calcium ionophore ionomycin (I) to activate lymphocytes obtained from popliteal lymph nodes (DLN) draining an MCA-105 footpad tumor. The adoptive transfer of B/I-stimulated DLN cells eradicated MCA-105 pulmonary metastases. These lymphocytes do not require concomitant IL-2 administration to mediate regression of lung metastases. Three days after intrasplenic injection of tumor cells and splenectomy, mice were given iv injections of B/I-stimulated DLN cells. Adoptive immunotherapy with these cells induced regression of established liver metastases. In an intradermal tumor model, the adoptive transfer of B/I-stimulated MCA-105 DLN cells cured mice of MCA-105 intradermal (id) tumors, but did not induce regression of MCA-206 tumors. Mice cured of MCA-105 id tumors were protected against MCA-105, but not MCA-203, tumor challenge in the footpad 7 weeks after adoptive immunotherapy.
Authors:
T M Tuttle; K P Bethke; T H Inge; C W McCrady; G R Pettit; H D Bear
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of surgical research     Volume:  52     ISSN:  0022-4804     ISO Abbreviation:  J. Surg. Res.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-10-22     Completed Date:  1992-10-22     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  543-8     Citation Subset:  IM    
Affiliation:
Department of Surgery, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / pharmacology*
Bryostatins
Female
Immunotherapy, Adoptive
Lactones / pharmacology*
Liver Neoplasms, Experimental / secondary
Lung Neoplasms / secondary
Lymphocyte Activation / drug effects*
Macrolides
Mice
Mice, Inbred C57BL
Neoplasms, Experimental / immunology,  therapy*
T-Lymphocytes / immunology*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Bryostatins; 0/Lactones; 0/Macrolides; 83314-01-6/bryostatin 1

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