Document Detail


Brush border myosin heavy chain phosphorylation is regulated by calcium and calmodulin.
MedLine Citation:
PMID:  3026852     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Myosin from chicken intestinal brush borders is phosphorylated on its heavy chains at threonine by a kinase isolated from brush borders. In contrast to other heavy chain kinases, the brush border kinase activity is dependent on calcium and calmodulin. The partially purified preparation also phosphorylated myosin on its light chains at serine, but in a calmodulin-independent manner. Phosphorylation of the light chains in the absence of calmodulin or both heavy and light chains in the presence of calmodulin activated its actin-activated ATPase activity about 10-fold, to about 50 nmol/min per mg.
Authors:
J P Rieker; H Swanljung-Collins; J Montibeller; J H Collins
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  FEBS letters     Volume:  212     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1987 Feb 
Date Detail:
Created Date:  1987-03-23     Completed Date:  1987-03-23     Revised Date:  2012-06-07    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  154-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / metabolism
Animals
Calcium / pharmacology*
Calcium-Calmodulin-Dependent Protein Kinases*
Calmodulin / pharmacology*
Chickens
Intestines
Microvilli / enzymology*
Myosins / metabolism*
Phosphorylation
Phosphotransferases / isolation & purification,  metabolism*
Protozoan Proteins
Grant Support
ID/Acronym/Agency:
5T32 HL07557/HL/NHLBI NIH HHS; GM32567/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Calmodulin; 0/Protozoan Proteins; 7440-70-2/Calcium; EC 2.7.-/Phosphotransferases; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases; EC 2.7.11.7/myosin-heavy-chain kinase; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.4.1/Myosins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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