Document Detail


Brucella ribosomal protein L7/L12 is a major component in the antigenicity of brucellin INRA for delayed-type hypersensitivity in brucella-sensitized guinea pigs.
MedLine Citation:
PMID:  7960115     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A delayed-type hypersensitivity (DTH) reaction in the course of brucellosis in humans and animals can be revealed by the brucellin INRA (Brucellergen) skin test. Brucellergen is composed of more than 20 proteins of different molecular weights. A 12-kDa protein eliciting DTH in Brucella melitensis Rev1-sensitized guinea pigs was found to be a significant component for the allergenic properties of Brucellergen. Sequencing of the gene encoding this protein identified it as the L7/L12 ribosomal protein. The L7/L12 gene of B. melitensis was amplified by PCR and subcloned in the Escherichia coli pQE30 plasmid. The resulting recombinant protein did not produce a DTH reaction in sensitized animals. It was used to raise specific antibodies in a rabbit. Affinity chromatography with these antibodies was used to isolate a single protein from Brucellergen and from B. melitensis cytosol preparations which produced a DTH reaction in guinea pigs sensitized with B. melitensis Rev1. N-terminal amino acid sequencing of the protein confirmed that it was the L7/L12 ribosomal protein. This is the first complete report on the involvement of a defined bacterial ribosomal protein in the DTH response of animals infected with intracellularly multiplying bacteria.
Authors:
G Bachrach; M Banai; S Bardenstein; G Hoida; A Genizi; H Bercovier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  62     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1994-12-29     Completed Date:  1994-12-29     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5361-6     Citation Subset:  IM    
Affiliation:
Department of Clinical Microbiology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
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MeSH Terms
Descriptor/Qualifier:
Allergens / immunology*
Animals
Antigens, Bacterial / chemistry,  immunology*
Base Sequence
Brucella melitensis / genetics
Brucellosis / immunology*
Escherichia coli Proteins
Genes, Bacterial / genetics
Guinea Pigs
Hypersensitivity, Delayed / immunology*
Male
Molecular Sequence Data
Ribosomal Proteins / genetics,  immunology*,  isolation & purification
Skin Tests
Chemical
Reg. No./Substance:
0/Allergens; 0/Antigens, Bacterial; 0/Escherichia coli Proteins; 0/Ribosomal Proteins; 0/rplL protein, E coli; 11028-17-4/brucellin; 70815-33-7/ribosomal protein L7-L12
Comments/Corrections

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