Document Detail


Brucella abortus requires the heme transporter BhuA for maintenance of chronic infection in BALB/c mice.
MedLine Citation:
PMID:  17709407     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The gene annotated BAB2_1150 in the Brucella abortus 2308 genome sequence is predicted to encode a homolog of the well-characterized heme transporter ShuA of Shigella dysenteriae and accordingly has been given the designation bhuA (Brucella heme utilization). Phenotypic analysis of an isogenic bhuA mutant derived from B. abortus 2308 verified that there is a link between BhuA and the ability of the parent strain to use heme as an iron source in in vitro assays. Maximum expression of bhuA in B. abortus 2308 is observed during stationary phase when this strain in cultivated in low-iron minimal medium, and a comparison of the growth characteristics of the B. abortus bhuA mutant and 2308 in this medium suggested that heme serves as an important iron source for the parent strain during stationary phase. The B. abortus bhuA mutant HR1703 exhibits significant attenuation in cultured murine macrophages compared to strain 2308, and unlike its parent strain, the B. abortus bhuA mutant is unable to maintain a chronic spleen infection in experimentally infected BALB/c mice. These experimental findings suggest that heme and/or heme-containing proteins represent important iron sources for B. abortus 2308 during its residence in the mammalian host and that BhuA is required for efficient utilization of these iron sources.
Authors:
James T Paulley; Eric S Anderson; R Martin Roop
Related Documents :
23135757 - Comparison of different phenotypic assays for the detection of extended-spectrum β-lac...
24871187 - The geometrid moths of ethiopia i: tribes pseudoterpnini and comibaenini (lepidoptera: ...
24243967 - Agromyces iriomotensis sp. nov. and agromyces subtropicus sp. nov., isolated from soil.
23748897 - Aliidiomarina sanyensis sp. nov., a hexabromocyclododecane assimilating bacterium from ...
10388547 - Laboratory screening of nematodes isolated from south australia for potential as biocon...
9056017 - Alterations in the gyra subunit of dna gyrase and the parc subunit of topoisomerase iv ...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-08-20
Journal Detail:
Title:  Infection and immunity     Volume:  75     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-19     Completed Date:  2007-11-29     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5248-54     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, East Carolina University School of Medicine, 600 Moye Boulevard, Greenville, NC 27834, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / genetics,  physiology*
Brucella abortus / genetics,  pathogenicity*
Brucellosis / microbiology*
Cells, Cultured
Colony Count, Microbial
Female
Gene Deletion
Heme / metabolism
Iron / metabolism
Macrophages, Peritoneal / microbiology
Membrane Transport Proteins / genetics,  physiology*
Mice
Mice, Inbred BALB C
Mutagenesis, Insertional
Spleen / microbiology
Virulence Factors / genetics,  physiology*
Grant Support
ID/Acronym/Agency:
AI63516/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/BhuA protein, Brucella abortus; 0/Membrane Transport Proteins; 0/Virulence Factors; 14875-96-8/Heme; 7439-89-6/Iron
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Coxiella burnetii inhibits activation of host cell apoptosis through a mechanism that involves preve...
Next Document:  Bacillus anthracis exosporium protein BclA affects spore germination, interaction with extracellular...