Document Detail


Brucella abortus deficient in copper/zinc superoxide dismutase is virulent in BALB/c mice.
MedLine Citation:
PMID:  1584004     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The gene encoding the Cu/Zn superoxide dismutase (SOD) of Brucella abortus strain 2308 was identified in a Brucella genomic library utilizing a combination of Western blotting and native gel electrophoresis. The Cu/Zn SOD gene was inactivated in vitro by ligation of a kanamycin resistance gene into the open reading frame encoding SOD. The plasmid born construct was introduced back into B. abortus by electroporation. Replacement of the wild-type Cu/Zn SOD by recombination was demonstrated by showing that both the KnR gene and the Cu/Zn SOD gene hybridized to the same band in a Southern analysis of genomic DNA. In addition, KnR strains were deficient in Cu/Zn SOD activity as assessed by lack of Cu/Zn SOD activity on a native gel and by lack of reactivity with specific serum in a Western analysis. Either strain 2308 or the Cu/Zn SOD deficient mutant injected intraperitoneally into BALB/c mice, exhibited no differences in their ability to colonize the spleen at 7 and 28 days post-inoculation. Thus, the inability to produce Cu/Zn SOD by B. abortus does not significantly impair its virulence in mice.
Authors:
E Latimer; J Simmers; N Sriranganathan; R M Roop; G G Schurig; S M Boyle
Related Documents :
24675854 - Draft genome sequence of the antarctic polyextremophile nesterenkonia sp. strain an1.
12757944 - Involvement of enterobactin in norepinephrine-mediated iron supply from transferrin to ...
23274994 - Diaminobutyricimonas aerilata gen. nov., sp. nov., a novel member of the family microba...
24091604 - Streptomyces fukangensis sp. nov., a novel alkaliphilic actinomycete isolated from a sa...
2655534 - Proportion of beta-d-glucuronidase-negative escherichia coli in human fecal samples.
3290184 - Trends in the epidemiology and antibiotic resistance of clinical staphylococcus strains...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Microbial pathogenesis     Volume:  12     ISSN:  0882-4010     ISO Abbreviation:  Microb. Pathog.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-06-18     Completed Date:  1992-06-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8606191     Medline TA:  Microb Pathog     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  105-13     Citation Subset:  IM    
Affiliation:
Department of Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Southern
Blotting, Western
Brucella abortus / enzymology,  genetics,  pathogenicity*
Brucellosis / microbiology
Cloning, Molecular
Mice
Mice, Inbred BALB C
Mutation
Superoxide Dismutase / deficiency,  genetics,  metabolism*
Virulence / genetics
Chemical
Reg. No./Substance:
EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A portable device for checking equipment and programs of detecting flutter and fibrillation of heart...
Next Document:  Effect of Legionella pneumophila cytotoxic protease on human neutrophil and monocyte function.