Document Detail

Brucella abortus deficient in copper/zinc superoxide dismutase is virulent in BALB/c mice.
MedLine Citation:
PMID:  1584004     Owner:  NLM     Status:  MEDLINE    
The gene encoding the Cu/Zn superoxide dismutase (SOD) of Brucella abortus strain 2308 was identified in a Brucella genomic library utilizing a combination of Western blotting and native gel electrophoresis. The Cu/Zn SOD gene was inactivated in vitro by ligation of a kanamycin resistance gene into the open reading frame encoding SOD. The plasmid born construct was introduced back into B. abortus by electroporation. Replacement of the wild-type Cu/Zn SOD by recombination was demonstrated by showing that both the KnR gene and the Cu/Zn SOD gene hybridized to the same band in a Southern analysis of genomic DNA. In addition, KnR strains were deficient in Cu/Zn SOD activity as assessed by lack of Cu/Zn SOD activity on a native gel and by lack of reactivity with specific serum in a Western analysis. Either strain 2308 or the Cu/Zn SOD deficient mutant injected intraperitoneally into BALB/c mice, exhibited no differences in their ability to colonize the spleen at 7 and 28 days post-inoculation. Thus, the inability to produce Cu/Zn SOD by B. abortus does not significantly impair its virulence in mice.
E Latimer; J Simmers; N Sriranganathan; R M Roop; G G Schurig; S M Boyle
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Microbial pathogenesis     Volume:  12     ISSN:  0882-4010     ISO Abbreviation:  Microb. Pathog.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-06-18     Completed Date:  1992-06-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8606191     Medline TA:  Microb Pathog     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  105-13     Citation Subset:  IM    
Department of Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061.
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MeSH Terms
Blotting, Southern
Blotting, Western
Brucella abortus / enzymology,  genetics,  pathogenicity*
Brucellosis / microbiology
Cloning, Molecular
Mice, Inbred BALB C
Superoxide Dismutase / deficiency,  genetics,  metabolism*
Virulence / genetics
Reg. No./Substance:
EC Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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