| Brucella abortus deficient in copper/zinc superoxide dismutase is virulent in BALB/c mice. | |
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MedLine Citation:
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PMID: 1584004 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The gene encoding the Cu/Zn superoxide dismutase (SOD) of Brucella abortus strain 2308 was identified in a Brucella genomic library utilizing a combination of Western blotting and native gel electrophoresis. The Cu/Zn SOD gene was inactivated in vitro by ligation of a kanamycin resistance gene into the open reading frame encoding SOD. The plasmid born construct was introduced back into B. abortus by electroporation. Replacement of the wild-type Cu/Zn SOD by recombination was demonstrated by showing that both the KnR gene and the Cu/Zn SOD gene hybridized to the same band in a Southern analysis of genomic DNA. In addition, KnR strains were deficient in Cu/Zn SOD activity as assessed by lack of Cu/Zn SOD activity on a native gel and by lack of reactivity with specific serum in a Western analysis. Either strain 2308 or the Cu/Zn SOD deficient mutant injected intraperitoneally into BALB/c mice, exhibited no differences in their ability to colonize the spleen at 7 and 28 days post-inoculation. Thus, the inability to produce Cu/Zn SOD by B. abortus does not significantly impair its virulence in mice. |
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Authors:
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E Latimer; J Simmers; N Sriranganathan; R M Roop; G G Schurig; S M Boyle |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Microbial pathogenesis Volume: 12 ISSN: 0882-4010 ISO Abbreviation: Microb. Pathog. Publication Date: 1992 Feb |
Date Detail:
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Created Date: 1992-06-18 Completed Date: 1992-06-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8606191 Medline TA: Microb Pathog Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 105-13 Citation Subset: IM |
Affiliation:
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Department of Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Southern Blotting, Western Brucella abortus / enzymology, genetics, pathogenicity* Brucellosis / microbiology Cloning, Molecular Mice Mice, Inbred BALB C Mutation Superoxide Dismutase / deficiency, genetics, metabolism* Virulence / genetics |
| Chemical | |
Reg. No./Substance:
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EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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