Document Detail


Brown fat-specific mitochondrial uncoupling protein in adipose tissues of newborn reindeer.
MedLine Citation:
PMID:  2058749     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reindeer inhabit a severe arctic or subarctic environment, with the young born in early spring under adverse weather conditions. The extreme northern climate imposes a major thermal challenge to the newborn, and in the present study we have examined fetal, neonatal, and young (from 2 wk before birth to 16 mo postpartum) semidomesticated reindeer from northern Finland for the presence of thermogenic brown adipose tissue. Adipose tissues were removed, mitochondria were prepared, and the proteins were separated by molecular weight and blotted onto nitrocellulose membranes. The membranes were then probed for the presence of the 32,000-relative molecular weight mitochondrial uncoupling protein (UCP) unique to brown fat by use of a rabbit anti-(ground squirrel UCP) serum. Immunoreactivity at the molecular weight characteristic of UCP was present in perirenal, abdominal, inter(pre)scapular, sternal, intralumbar, vertebral, tracheal, inguinal, and omental-mesenteral adipose tissues of newborn reindeer (0-2 days of age). No immunoreactivity was detected in coronary adipose tissue. UCP was found at high levels in interscapular and perirenal adipose tissues of fetal reindeer at 2 wk before birth. Although the protein was present during the first few days postpartum, little immunoreactivity was found at 1 mo of age, and none was evident by 2 mo. UCP and its mRNA were also apparent in perirenal adipose tissue of the newborn of another species of Cervidae, the red deer. It is concluded, on the basis of the immunologic identification of UCP, that most adipose tissues of newborn reindeer represent functional brown fat but that there is a subsequent conversion to white adipose tissue by the 2nd mo of life.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
P Soppela; M Nieminen; S Saarela; J S Keith; J N Morrison; F Macfarlane; P Trayhurn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  260     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1991 Jun 
Date Detail:
Created Date:  1991-08-01     Completed Date:  1991-08-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R1229-34     Citation Subset:  IM    
Affiliation:
Reindeer Research, Finnish Game and Fisheries Research Institute, Rovaniemi.
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / chemistry*,  metabolism
Aging / metabolism
Animals
Animals, Newborn / metabolism*
Base Sequence
Carrier Proteins*
DNA Probes
Immunoblotting
Ion Channels
Membrane Proteins / analysis*,  genetics,  metabolism
Mitochondrial Proteins
Molecular Sequence Data
RNA, Messenger / genetics,  metabolism
Reindeer / metabolism*
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/DNA Probes; 0/Ion Channels; 0/Membrane Proteins; 0/Mitochondrial Proteins; 0/RNA, Messenger; 0/mitochondrial uncoupling protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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