| Bronchogenic and alveologenic tumors in mice induced by N-nitrosopiperidine. | |
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MedLine Citation:
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PMID: 20651851 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to explore the histogenesis and carcinogenesis of pulmonary cancer induced by N-nitrosopiperidine (NPIP) in mice. NPIP is a form of N-nitrosamine found in tobacco smoke, which has been shown to be a genotoxic chemical as well as a mutagenic compound for inducing chromosome aberrations and severe clastogenicity. In this study, 80 BALB/C strain mice were injected with 0.2 mmol/kg NPIP intraperitoneally for 8 weeks, and experiments were conducted for a further 16 weeks. For the control group, 40 mice were injected with an equal volume of 0.9% NaCl. Pulmonary tissues and tumors in the NPIP-treated group were examined by light microscopy and transmission electron microscopy and compared with the control group at 4-week intervals. The mRNA levels of p53 (mutant), bcl-2, c-myc, ras, and subunits of telomerase - telomerase reverse transcriptase (TERT) and an RNA component, TR - were assayed by mPCR or RT-PCR. Twenty-two mice in the experimental group were found to develop pulmonary tumors, but none in the control group. All tumors found in the experimental group originated from alveolar type II epithelial cells. In addition, 6 of the 22 mice also developed tumors of bronchogenic origin. The expression of p53, bcl-2, c-myc, ras, and the subunits of telomerase were found to increase in all pulmonary tissues and tumors formed thereafter upon NPIP treatment. In summary, NPIP-induced mouse lung tumors exhibited morphological changes during carcinogenesis, which may be the consequence of overexpression of some genes associated with the development of carcinoma and changes in subunits of telomerase. This mouse model of lung tumor formation may be a useful tool to delineate the histogenesis and carcinogenesis of human pulmonary cancer. |
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Authors:
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Xiao-Yuan Xie; Jian Shen; Li-Yan Xu; En-Min Li; Zhong-Ying Shen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry and cell biology = Biochimie et biologie cellulaire Volume: 88 ISSN: 1208-6002 ISO Abbreviation: Biochem. Cell Biol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-23 Completed Date: 2010-12-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8606068 Medline TA: Biochem Cell Biol Country: Canada |
Other Details:
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Languages: eng Pagination: 775-82 Citation Subset: IM |
Affiliation:
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Department of Tumor Medicine, The First Affiliated Hospital, Shantou University Medical College, Shantou, P.R. China. starsea65@163.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoma
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chemically induced,
genetics,
pathology,
ultrastructure Animals Carcinoma, Bronchogenic / chemically induced*, genetics, pathology, ultrastructure Carcinoma, Squamous Cell / chemically induced, genetics, pathology, ultrastructure Female Gene Expression Regulation, Neoplastic Genes, bcl-2 Genes, myc Genes, p53 Genes, ras Lung Neoplasms / chemically induced*, genetics, pathology, ultrastructure Male Mice Mice, Inbred BALB C Nitrosamines* Pulmonary Alveoli / drug effects, metabolism, pathology, ultrastructure Telomerase / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Nitrosamines; 100-75-4/N-nitrosopiperidine; EC 2.7.7.49/Telomerase; EC 2.7.7.49/Tert protein, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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