Document Detail

Bronchoalveolar lavage neutrophilia in asthmatic and healthy volunteers after controlled exposure to ozone and filtered purified air.
MedLine Citation:
PMID:  7988196     Owner:  NLM     Status:  MEDLINE    
STUDY OBJECTIVE: To characterize the pulmonary response of asthmatic and healthy nonsmoking adult men to 0.20 ppm ozone by controlled chamber exposure. DESIGN: A prospective, crossover study of five atopic asthmatic and five normal subjects randomly exposed to ozone and filtered purified air (FPA) for 6 h, consisting of 30-min alternating periods of rest and moderate exercise. The two exposures were separated by at least 30 days. SETTING: A controlled exposure in a stainless steel chamber. PATIENTS: Five atopic asthmatic and five normal subjects between 18 and 45 years of age. Treatment with medications was withheld from asthmatics prior to the exposures. All subjects were nonsmokers. INTERVENTIONS: Symptoms were assessed throughout the exposures. Pulmonary function was measured at baseline, hourly throughout an exposure, and after an exposure. Bronchoalveolar lavage (BAL) was performed 18 h after the completion of an exposure. The BAL fluid (BALF) was analyzed for cell count and differential; the cell-free supernatant was analyzed for albumin, tumor necrosis factor (TNF), interleukin 1 (IL-1), interleukin 6 (IL-6), and interleukin 8 (IL-8). RESULTS: There were statistically significant increases in IL-8 levels, as well as percent polymorphonuclear neutrophils (PMNs) and PMNs per milliliter of lavage in asthmatics exposed to ozone as compared with the same asthmatics exposed to FPA and the same normal subjects exposed to ozone and FPA. Interleukin 6 was also significantly increased in asthmatics exposed to ozone. The BALF albumin, TNF, and IL-1 levels were not significantly different among the four groups. There were no differences between asthmatics and healthy controls exposed to ozone or FPA in baseline to postexposure FEV1, FVC, FEV1/FVC, and sRaw. CONCLUSIONS: We conclude that asthmatics exposed to ozone develop a significant BALF neutrophilia and increased levels of the cytokines, IL-8 and IL-6. These BALF findings occur even though the level of ozone exposure was not significant enough to reduce pulmonary function.
M A Basha; K B Gross; C J Gwizdala; A H Haidar; J Popovich
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Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chest     Volume:  106     ISSN:  0012-3692     ISO Abbreviation:  Chest     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-01-10     Completed Date:  1995-01-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0231335     Medline TA:  Chest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1757-65     Citation Subset:  AIM; IM    
Department of Internal Medicine, Henry Ford Health Sciences Center, Detroit, Mich.
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MeSH Terms
Albumins / analysis
Asthma / metabolism,  pathology*,  physiopathology
Bronchial Provocation Tests
Bronchoalveolar Lavage Fluid / chemistry,  cytology*
Cell Count
Cross-Over Studies
Forced Expiratory Volume
Interleukins / analysis
Maximal Midexpiratory Flow Rate
Middle Aged
Neutrophils / pathology*
Ozone / administration & dosage,  pharmacology*
Prospective Studies
Tumor Necrosis Factor-alpha / analysis
Vital Capacity
Reg. No./Substance:
0/Albumins; 0/Interleukins; 0/Tumor Necrosis Factor-alpha; 10028-15-6/Ozone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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