| Bronchial epithelial cells respond to insulin and insulin-like growth factor-I as a chemoattractant. | |
| | |
MedLine Citation:
|
PMID: 2189458 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Migration of epithelial cells to cover areas of injury is thought to be important in the repair process following airway insult. Insulin is reported to be a growth factor for bronchial epithelial cells, and growth factors have been known to be chemotactic for many types of cells. Thus, we hypothesized that insulin may be a chemoattractant for bronchial epithelial cells. To evaluate this, we prepared bronchial epithelial cells and measured their chemotactic activity toward insulin. Bronchial epithelial cells were isolated by overnight digestion with bacterial protease, filtered through 100-microns nitex mesh, and then cultured at 1 x 10(6) cells/ml in tissue culture dishes in medium 199 supplemented with transferrin, insulin, epidermal growth factor, hydrocortisone, antibiotics, and 10% FCS for 3 d. The cultured cells were rinsed twice to remove supplements, trypsinized and resuspended at 1 x 10(6) cells/ml in medium 199 without supplements, and used as the cell source for chemotaxis. Chemotactic activity of bronchial epithelial cells was measured by the blindwell chamber technique using 8-microns Nuclepore filter membranes coated with 0.1% gelatin. The cells were added to the top wells in a 48-multiwell chamber with insulin in the bottom wells and incubated for 6 h at 37 degrees C, 5% CO2. Bronchial epithelial cells migrated in response to insulin in a dose-dependent manner up to an optimal dose of insulin, 100 micrograms/ml, and decreased at higher concentrations. The number of migrated cells per 10 high power fields was 33.7 +/- 1.9 at the optimum and 3.7 +/- 0.7 without insulin (P less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS) |
| | |
Authors:
|
S Shoji; R F Ertl; J Linder; S Koizumi; W C Duckworth; S I Rennard |
Related Documents
:
|
2430458 - Induction of hla-dr antigen expression in human endometrial epithelial cells in vitro b... 18691068 - Regulation of mucous cell metaplasia in bronchial asthma. 2579838 - Localization of keratin in the cells of the cornea in aphakia and normal mouse embryos. 2606218 - Corneal epithelial cell movement in humans. 9497028 - Heterogeneity of dna content and expression of cell cycle genes in axenically growing e... 18690648 - Disialoganglioside gd3 synthase expression recruits membrane transglutaminase 2 during ... |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: American journal of respiratory cell and molecular biology Volume: 2 ISSN: 1044-1549 ISO Abbreviation: Am. J. Respir. Cell Mol. Biol. Publication Date: 1990 Jun |
Date Detail:
|
Created Date: 1990-07-06 Completed Date: 1990-07-06 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8917225 Medline TA: Am J Respir Cell Mol Biol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 553-7 Citation Subset: IM |
Affiliation:
|
Department of Internal Medicine, University of Nebraska Medical Center, Omaha 68198-2465. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bronchi / physiology* Cattle Cell Membrane / drug effects Cells, Cultured Chemotaxis* Dose-Response Relationship, Drug Epithelium / physiology Insulin / administration & dosage, pharmacology* Insulin-Like Growth Factor I / administration & dosage, pharmacology* Insulin-Like Growth Factor II / administration & dosage, pharmacology Somatomedins / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Somatomedins; 11061-68-0/Insulin; 67763-96-6/Insulin-Like Growth Factor I; 67763-97-7/Insulin-Like Growth Factor II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Expression of cell-specific markers for alveolar epithelium in fetal rat lung.
Next Document: Applications of magnetic resonance imaging to gynecology.