Document Detail


Bromelain purification through unconventional aqueous two-phase system (PEG/ammonium sulphate).
MedLine Citation:
PMID:  22763776     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This paper focuses on the feasibility of unconventional aqueous two-phase systems for bromelain purification from pineapple processing waste. The main difference in comparison with conventional systems is the integration of the liquid-liquid extraction technique with fractional precipitation, which can decrease the protein content with no loss of biological activity by removing of unwanted molecules. The analysis of the results was based on the response surface methodology and revealed that the use of the desirability optimisation methodology (DOM) was necessary to achieve higher purification factor values and greater bromelain recovery. The use of DOM yielded an 11.80-fold purification factor and 66.38 % biological activity recovery using poly(ethylene glycol) (PEG) with a molar mass of 4,000, 10.86 % PEG concentration (m/m) and 36.21 % saturation of ammonium sulphate.
Authors:
D F Coelho; E Silveira; A Pessoa Junior; E B Tambourgi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-5
Journal Detail:
Title:  Bioprocess and biosystems engineering     Volume:  -     ISSN:  1615-7605     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088505     Medline TA:  Bioprocess Biosyst Eng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Sala C205, Bloco C, Departamento de Engenharia de Sistemas Químicos, Faculdade de Engenharia Química, Universidade Estadual de Campinas (UNICAMP), Cidade Universitária "Zeferino Vaz", Av. Albert Einstein, 500, Campinas, São Paulo, CEP 13083-852, Brazil, dfcoelho@feq.unicamp.br.
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