Document Detail


Broad tuning of the human bitter taste receptor hTAS2R46 to various sesquiterpene lactones, clerodane and labdane diterpenoids, strychnine, and denatonium.
MedLine Citation:
PMID:  17595105     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sesquiterpene lactones are a major class of natural bitter compounds occurring in vegetables and culinary herbs as well as in aromatic and medicinal plants, where they often represent the main gustatory and pharmacologically active component. Investigations on sesquiterpene lactones have mainly focused on their bioactive potential rather than on their sensory properties. In the present study, we report about the stimulation of heterologously expressed human bitter taste receptors, hTAS2Rs, by the bitter sesquiterpene lactone herbolide D. A specific response to herbolide D was observed i.a. for hTAS2R46, a so far orphan bitter taste receptor without any known ligand. By further investigation of its agonist pattern, we characterized hTAS2R46 as a bitter receptor broadly tuned to sesquiterpene lactones and to clerodane and labdane diterpenoids as well as to the unrelated bitter substances strychnine and denatonium.
Authors:
Anne Brockhoff; Maik Behrens; Alberto Massarotti; Giovanni Appendino; Wolfgang Meyerhof
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-27
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  55     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-19     Completed Date:  2007-10-09     Revised Date:  2007-10-18    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6236-43     Citation Subset:  IM    
Affiliation:
Department of Molecular Genetics, German Institute of Human Nutrition Potsdam-Rehbruecke, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany.
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MeSH Terms
Descriptor/Qualifier:
Diterpenes / pharmacology*
Diterpenes, Clerodane / pharmacology
Dose-Response Relationship, Drug
Gene Expression / drug effects
Humans
Lactones / pharmacology
Quaternary Ammonium Compounds / pharmacology*
Receptors, G-Protein-Coupled / drug effects*,  genetics
Sesquiterpenes / pharmacology*
Strychnine / pharmacology*
Chemical
Reg. No./Substance:
0/Diterpenes; 0/Diterpenes, Clerodane; 0/Lactones; 0/Quaternary Ammonium Compounds; 0/Receptors, G-Protein-Coupled; 0/Sesquiterpenes; 0/labdane; 0/taste receptors, type 2; 3734-33-6/denatonium benzoate; 57-24-9/Strychnine

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