| Broad reactive monoclonal antibodies for rapid identification of enteroviruses show cross-reactivity with chikungunya virus infected cells. | |
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MedLine Citation:
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PMID: 20614726 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the past decade, enterovirus 71 (EV71) and chikungunya (CHIK) virus have re-emerged periodically causing serious public health problems in Malaysia, since their first emergence in 1997 and 1998 respectively. This study demonstrates that CHIK virus causes similar patterns of cytopathic effect in cultured Vero cells as some enteroviruses. They also show positive cross-reaction on direct immunofluorescence staining using monoclonal antibodies meant for typing enteroviruses. Without adequate clinical and epidemiological information for correlation, CHIK virus isolated from patients with acute febrile rash can be wrongly reported as untypeable enterovirus due to its cross-reactivity with commercial pan-enterovirus monoclonal antibodies. This is due to the diagnostic laboratory being unaware of such cross-reactions as it has not been reported previously. Final identification of the virus could be determined with specific antibodies or molecular typing using specific oligonucleotide primers for the CHIK virus. |
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Authors:
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A H Khairul; Y K Chem; C Keniscope; J Rosli; S Hassan; Jais Mat; K B Chua |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Malaysian journal of pathology Volume: 32 ISSN: 0126-8635 ISO Abbreviation: Malays J Pathol Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-07-09 Completed Date: 2010-08-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8101177 Medline TA: Malays J Pathol Country: Malaysia |
Other Details:
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Languages: eng Pagination: 49-52 Citation Subset: IM |
Affiliation:
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The National Public Health Laboratory, Ministry of Health of Malaysia, Sungai Buloh, Selangor, Malaysia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alphavirus Infections
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diagnosis* Animals Antibodies, Monoclonal / diagnostic use* Antibodies, Viral / immunology* Antibody Specificity Cercopithecus aethiops Chikungunya virus / immunology, isolation & purification* Cross Reactions Enterovirus / immunology, isolation & purification* Enterovirus Infections / diagnosis* Humans Reverse Transcriptase Polymerase Chain Reaction Vero Cells |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antibodies, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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