| Broad and potent neutralizing antibodies from an African donor reveal a new HIV-1 vaccine target. | |
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MedLine Citation:
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PMID: 19729618 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Broadly neutralizing antibodies (bNAbs), which develop over time in some HIV-1-infected individuals, define critical epitopes for HIV vaccine design. Using a systematic approach, we have examined neutralization breadth in the sera of about 1800 HIV-1-infected individuals, primarily infected with non-clade B viruses, and have selected donors for monoclonal antibody (mAb) generation. We then used a high-throughput neutralization screen of antibody-containing culture supernatants from about 30,000 activated memory B cells from a clade A-infected African donor to isolate two potent mAbs that target a broadly neutralizing epitope. This epitope is preferentially expressed on trimeric Envelope protein and spans conserved regions of variable loops of the gp120 subunit. The results provide a framework for the design of new vaccine candidates for the elicitation of bNAb responses. |
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Authors:
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Laura M Walker; Sanjay K Phogat; Po-Ying Chan-Hui; Denise Wagner; Pham Phung; Julie L Goss; Terri Wrin; Melissa D Simek; Steven Fling; Jennifer L Mitcham; Jennifer K Lehrman; Frances H Priddy; Ole A Olsen; Steven M Frey; Phillip W Hammond; ; Stephen Kaminsky; Timothy Zamb; Matthew Moyle; Wayne C Koff; Pascal Poignard; Dennis R Burton |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-09-03 |
Journal Detail:
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Title: Science (New York, N.Y.) Volume: 326 ISSN: 1095-9203 ISO Abbreviation: Science Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-10-09 Completed Date: 2009-10-19 Revised Date: 2012-04-25 |
Medline Journal Info:
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Nlm Unique ID: 0404511 Medline TA: Science Country: United States |
Other Details:
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Languages: eng Pagination: 285-9 Citation Subset: IM |
Affiliation:
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Department of Immunology and Microbial Science and IAVI Neutralizing Antibody Center, Scripps Research Institute, La Jolla, CA 92037, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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AIDS Vaccines
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immunology* Africa South of the Sahara Antibodies, Monoclonal / immunology* B-Lymphocyte Subsets / immunology Epitopes / immunology HIV Antibodies / biosynthesis, immunology* HIV Envelope Protein gp120 / chemistry, immunology* HIV Envelope Protein gp41 / immunology HIV Infections / immunology* HIV-1 / immunology* Humans Immunologic Memory Lymphocyte Activation Neutralization Tests Peptide Fragments / immunology Protein Multimerization Recombinant Proteins / immunology |
| Grant Support | |
ID/Acronym/Agency:
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AI33292/AI/NIAID NIH HHS; R01 AI033292-18/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/AIDS Vaccines; 0/Antibodies, Monoclonal; 0/Epitopes; 0/HIV Antibodies; 0/HIV Envelope Protein gp120; 0/HIV Envelope Protein gp41; 0/HIV envelope protein gp120 (305-321); 0/Peptide Fragments; 0/Recombinant Proteins; 0/gp120 protein, Human immunodeficiency virus 1; 0/gp41 protein, Human immunodeficiency virus 1 |
| Investigator | |
Investigator/Affiliation:
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G Miiro / ; J Serwanga / ; A Pozniak / ; D McPhee / ; O Manigart / ; L Mwananyanda / ; E Karita / ; A Inwoley / ; W Jaoko / ; J DeHovitz / ; L G Bekker / ; P Pitisuttithum / ; R Paris / ; S Allen / |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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