Document Detail

Broad neutralizing human monoclonal antibodies against influenza virus from vaccinated healthy donors.
MedLine Citation:
PMID:  19580789     Owner:  NLM     Status:  MEDLINE    
Human monoclonal antibodies (HuMAbs) prepared from patients with viral infections could provide information on human epitopes important for the development of vaccines as well as potential therapeutic applications. Through the fusion of peripheral blood mononuclear cells from a total of five influenza-vaccinated volunteers, with newly developed murine-human chimera fusion partner cells, named SPYMEG, we obtained 10 hybridoma clones stably producing anti-influenza virus antibodies: one for influenza A H1N1, four for influenza A H3N2 and five for influenza B. Surprisingly, most of the HuMAbs showed broad reactivity within subtype and four (two for H3N2 and two for B) showed broad neutralizing ability. Importantly, epitope mapping revealed that the two broad neutralizing antibodies to H3N2 derived from different donors recognized the same epitope located underneath the receptor-binding site of the hemagglutinin globular region that is highly conserved among H3N2 strains.
Ritsuko Kubota-Koketsu; Hiroyuki Mizuta; Masatoshi Oshita; Shoji Ideno; Mikihiro Yunoki; Motoki Kuhara; Naomasa Yamamoto; Yoshinobu Okuno; Kazuyoshi Ikuta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-04
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  387     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-07-31     Completed Date:  2009-08-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  180-5     Citation Subset:  IM    
Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.
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MeSH Terms
Amino Acid Sequence
Antibodies, Monoclonal / immunology*
Antibodies, Viral / immunology*
Epitope Mapping
Epitopes / immunology
Immunoglobulin G / immunology
Immunoglobulin Heavy Chains / immunology
Immunoglobulin Light Chains / immunology
Influenza A Virus, H1N1 Subtype / immunology*
Influenza A Virus, H3N2 Subtype / immunology*
Influenza B virus / immunology*
Influenza Vaccines / immunology*
Middle Aged
Molecular Sequence Data
Neutralization Tests
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Viral; 0/Epitopes; 0/Immunoglobulin G; 0/Immunoglobulin Heavy Chains; 0/Immunoglobulin Light Chains; 0/Influenza Vaccines

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