| Broad neutralizing human monoclonal antibodies against influenza virus from vaccinated healthy donors. | |
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MedLine Citation:
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PMID: 19580789 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human monoclonal antibodies (HuMAbs) prepared from patients with viral infections could provide information on human epitopes important for the development of vaccines as well as potential therapeutic applications. Through the fusion of peripheral blood mononuclear cells from a total of five influenza-vaccinated volunteers, with newly developed murine-human chimera fusion partner cells, named SPYMEG, we obtained 10 hybridoma clones stably producing anti-influenza virus antibodies: one for influenza A H1N1, four for influenza A H3N2 and five for influenza B. Surprisingly, most of the HuMAbs showed broad reactivity within subtype and four (two for H3N2 and two for B) showed broad neutralizing ability. Importantly, epitope mapping revealed that the two broad neutralizing antibodies to H3N2 derived from different donors recognized the same epitope located underneath the receptor-binding site of the hemagglutinin globular region that is highly conserved among H3N2 strains. |
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Authors:
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Ritsuko Kubota-Koketsu; Hiroyuki Mizuta; Masatoshi Oshita; Shoji Ideno; Mikihiro Yunoki; Motoki Kuhara; Naomasa Yamamoto; Yoshinobu Okuno; Kazuyoshi Ikuta |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-04 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 387 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-07-31 Completed Date: 2009-08-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 180-5 Citation Subset: IM |
Affiliation:
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Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Amino Acid Sequence Antibodies, Monoclonal / immunology* Antibodies, Viral / immunology* Epitope Mapping Epitopes / immunology Female Humans Immunoglobulin G / immunology Immunoglobulin Heavy Chains / immunology Immunoglobulin Light Chains / immunology Influenza A Virus, H1N1 Subtype / immunology* Influenza A Virus, H3N2 Subtype / immunology* Influenza B virus / immunology* Influenza Vaccines / immunology* Male Middle Aged Molecular Sequence Data Neutralization Tests |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antibodies, Viral; 0/Epitopes; 0/Immunoglobulin G; 0/Immunoglobulin Heavy Chains; 0/Immunoglobulin Light Chains; 0/Influenza Vaccines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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