Document Detail


Broad-band ion accumulation with an internal source MALDI-FTICR-MS.
MedLine Citation:
PMID:  11140753     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new method is described which allows a broad mass range of ions from multiple MALDI events to be accumulated in an FIICR analyzer cell prior to detection. Signal intensity and signal-to-noise ratio are observed to grow in direct proportion to the number of laser shots, providing a substantial improvement to the analysis of low-level samples compared to more commonly used signal-averaging methods. In addition, calibrant ions desorbed from a separate sample spot can be added to the analyzer cell after an analyte population has been accumulated, providing an internal mass standard without the necessity of mixing calibrant and analyte. This method provides excellent mass accuracy by eliminating mass errors due to space charge effects.
Authors:
T H Mize; I J Amster
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Analytical chemistry     Volume:  72     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-01-03     Completed Date:  2001-01-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5886-91     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of Georgia, Athens 30602-2556, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Fourier Analysis
Molecular Sequence Data
Serum Albumin, Bovine / chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Chemical
Reg. No./Substance:
0/Serum Albumin, Bovine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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