| Bringing the excitement and motivation of research to students; Using inquiry and research-based learning in a year-long biochemistry laboratory: Part I-guided inquiry-purification and characterization of a fusion protein: Histidine tag, malate dehydrogenase, and green fluorescent protein. | |
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MedLine Citation:
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PMID: 21567851 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A successful laboratory experience provides the foundation for student success, creating active participation in the learning process. Here, we describe a new approach that emphasizes research, inquiry and problem solving in a year-long biochemistry experience. The first semester centers on the purification, characterization, and analysis of a novel fusion protein within a guided research experience. Throughout the semester, students gradually acquire skills as they are allowed to work independently. A fusion protein, malate dehydrogenase-green fluorescent protein with a histidine affinity tag (MGH), is used throughout the semester. The fusion protein allows for a high throughput analysis and is stable for duration of the semester. Students start with the purification and analysis of the plasmid DNA and end with an enzymatic analysis of MGH. As students take ownership of their experiments and choose two different chromatographic resins, they make many choices throughout the semester. Skills, motivation, confidence levels, and attitudes were assessed before and after the semester. Students achieved high levels of critical biochemical laboratory skills and critical thinking while increasing their confidence and motivation for working in a biochemical research setting. |
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Authors:
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Kristopher Knutson; Jennifer Smith; Mark A Wallert; Joseph J Provost |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology Volume: 38 ISSN: 1539-3429 ISO Abbreviation: Biochem Mol Biol Educ Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2011-05-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100970605 Medline TA: Biochem Mol Biol Educ Country: United States |
Other Details:
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Languages: eng Pagination: 317-23 Citation Subset: - |
Copyright Information:
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Copyright © 2010 International Union of Biochemistry and Molecular Biology, Inc. |
Affiliation:
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Department of Chemistry and the Biochemistry and Biotechnology Program, Minnesota State University Moorhead, Minnesota, Minnesota 56563. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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