Document Detail


Brief introduction to fluorescence correlation spectroscopy.
MedLine Citation:
PMID:  23276534     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Fluorescence correlation spectroscopy (FCS) measures rates of transport (diffusion coefficients, convection velocities) and chemical reactions (rate constants) in equilibrium or nonequilibrium steady-state systems without the need for a perturbation of the state of the system. The rates are extracted from a record of fluorescence fluctuations observed from an open laser-illuminated observation volume. In addition, FCS provides the number of measured fluorescent molecules in the observation volume and, therefore, their molecular brightness. This enables sensitive measurements of aggregation of the fluorescent system components. FCS is now used as a routine tool for studying systems in physics, chemistry, and biology. Recent extensions of the fluorescence fluctuation approach have included methods based on imaging and cross correlation. Because of the sensitivity and chemical specificity of fluorescence detection and also the ability to form a very small diffraction-limited detection volume, FCS is especially useful for studies of dynamic molecular processes in living biological cells. FCS provides a window on mesoscopic systems and is closely related to fluorescence methods for studying single molecules.
Authors:
Elliot L Elson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in enzymology     Volume:  518     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  11-41     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier Inc. All rights reserved.
Affiliation:
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, Campus Box 8231, St. Louis, Missouri, USA. Electronic address: elson@wustl.edu.
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