Document Detail

Bridging effect of recombinant human mannose-binding lectin in macrophage phagocytosis of Escherichia coli.
MedLine Citation:
PMID:  18284465     Owner:  NLM     Status:  MEDLINE    
Mannose-binding lectin (MBL) exists in the serum as a complex with MBL-associated serine protease (MASP). A recent paper described how MASP-free recombinant rat MBL stimulates the phagocytosis of Escherichia coli and Staphylococcus aureus by rat Kupffer cells through an increase in the level of a phagocytosis receptor. We have examined the effect of human MBL on the phagocytic action of human macrophages. Purified recombinant human MBL stimulated the phagocytosis of E. coli by THP-1 macrophages, leaving that of latex beads, apoptotic human cells, zymosan particles or S. aureus unchanged. This stimulatory effect was observed when either phagocytes or targets were preincubated with MBL. Furthermore, MBL bound to THP-1 macrophages as well as to E. coli, but not to S. aureus, through lipid A. These results indicated that human MBL in the absence of MASP stimulates macrophage phagocytosis of E. coli by bridging targets and phagocytes.
Akiko Shiratsuchi; Ikuko Watanabe; Jin-Sung Ju; Bok Luel Lee; Yoshinobu Nakanishi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-12
Journal Detail:
Title:  Immunology     Volume:  124     ISSN:  1365-2567     ISO Abbreviation:  Immunology     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-09-18     Completed Date:  2008-12-04     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  575-83     Citation Subset:  IM    
Graduate School of Medical Science, Kanazawa University, Shizenken, Kakuma-machi, Kanazawa, Ishikawa, Japan.
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MeSH Terms
Calreticulin / immunology
Cell Line
Escherichia coli / immunology*
Lipopolysaccharides / immunology
Macrophages / immunology*
Mannose-Binding Lectin / immunology*
Mannose-Binding Protein-Associated Serine Proteases / deficiency
Microscopy, Fluorescence
Phagocytosis / immunology*
Protein Binding / immunology
Recombinant Proteins / immunology
Reg. No./Substance:
0/Calreticulin; 0/Lipopolysaccharides; 0/Mannose-Binding Lectin; 0/Recombinant Proteins; EC 3.4.21.-/Mannose-Binding Protein-Associated Serine Proteases

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