| Breviscapine inhibits high glucose-induced proliferation and migration of cultured vascular smooth muscle cells of rats via suppressing the ERK1/2 MAPK signaling pathway. | |
| | |
MedLine Citation:
|
PMID: 22465949 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Aim:To investigate the influences of breviscapine, a flavonoid extracted from Erigeron breviscapus, on the proliferation and migration of vascular smooth muscle cells (VSMCs) cultured in a high glucose medium and the underlying mechanisms.Methods:VSMCs were isolated from thoracic aortas of male Sprague-Dawley rats and cultured in vitro. Cell proliferation was evaluated using Counting Kit-8 cell viability assay. Cell migration was evaluated using transwell migration assay and in vitro scratch assay. The expression and activity of protein kinase C-β2 (PKC-β2), extracellular signal-regulated kinase 1/2 (ERK1/2), p38 mitogen-activated protein kinase (p38), and JNK mitogen-activated protein kinase (JNK) were measured with Western blotting.Results:Exposure of VSMCs to a high glucose (25 mmol/L) medium significantly increased the proliferation and migration potential as compared to the control group. Pretreatment with breviscapine (65 μmol/L and 108 μmol/L) attenuated high glucose-enhanced proliferation and migration of VSMCs. Exposure of VSMCs to the high glucose medium activated both the PKC-β2 and ERK1/2 MAPK, but not the p38 and JNK MAPK. Pretreatment with breviscapine (65 μmol/L and 108 μmol/L) blocked high glucose-induced increase of the ERK1/2 activity, but not that of the PKC-β2 activity.Conclusion:Our study demonstrated that breviscapine ameliorates high glucose-induced proliferation and migration of VSMCs via inhibiting ERK1/2 MAPK signaling. |
| | |
Authors:
|
Meng He; Zhi-Min Xue; Juan Li; Bin-Quan Zhou |
Related Documents
:
|
11087679 - Purification of gsk-3 by affinity chromatography on immobilized axin. 12130649 - Activations of erk1/2 and jnk by transforming growth factor beta negatively regulate sm... 20820849 - Smad linker region phosphorylation in the regulation of extracellular matrix synthesis. 22311299 - Glucagon-like peptide 1 (glp-1) can reverse amp-activated protein kinase (ampk) and s6 ... 22718609 - Gsk3: a key target for the development of novel treatments for type 2 diabetes mellitus... 22415589 - Pentamethylquercetin generates beneficial effects in monosodium glutamate-induced obese... 17317139 - Crossing paths with notch in the hyper-network. 11087679 - Purification of gsk-3 by affinity chromatography on immobilized axin. 15319269 - Inhibition of rho-kinase leads to rapid activation of phosphatidylinositol 3-kinase/pro... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-4-02 |
Journal Detail:
|
Title: Acta pharmacologica Sinica Volume: - ISSN: 1745-7254 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
|
Created Date: 2012-4-2 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100956087 Medline TA: Acta Pharmacol Sin Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
The Department of Cardiology, Biomedical Research (Therapy) Center, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou 310016, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Non-genomic vasorelaxant effects of 17?-estradiol and progesterone in rat aorta are mediated by L-ty...
Next Document: Inhibition of hypoxia inducible factor-1? ameliorates lung injury induced by trauma and hemorrhagic ...