| Breath pentane: an indicator for early and continuous monitoring of lipid peroxidation in hepatic ischaemia-reperfusion injury. | |
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MedLine Citation:
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PMID: 19445060 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND OBJECTIVE: Lipid peroxidation plays an important role during liver ischaemia-reperfusion injury. Pentane in breath is often used as an index of lipid peroxidation. We observed the changes in levels of breath pentane during the lipid peroxidation process caused by liver ischaemia-reperfusion injury. METHODS: Ten male swine were anaesthetized with chloral hydrate 0.3-0.5 g kg(-1) min(-1). Total hepatic ischaemia was induced by occluding the portal inflow vessels. Ischaemia lasted 30 min followed by reperfusion for 180 min. Breath samples were sampled from the anaesthesia circuit and blood samples were collected from the inferior vena cava. Pentane concentrations in breath and blood were quantified by means of solid phase microextraction and gas chromatography-mass spectrography technique. RESULTS: Exhaled pentane concentrations (means +/- SE) increased markedly after reperfusion for 1 min (244.13 +/- 33.3 pmol l(-1)) and decreased gradually to initial levels after reperfusion for 60 min. Blood pentane concentrations (means +/- SE) increased significantly after reperfusion for 1 min (333.46 +/- 63.05 pmol l(-1)) and then decreased to basal level. Breath pentane concentrations showed a correlation with blood (r = 0.709, P < 0.05). CONCLUSION: Breath pentane analysis could provide early, rapid, noninvasive and continuous assessment of lipid peroxidation during hepatic ischaemia-reperfusion injury. |
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Authors:
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Peng Li; Guowang Xu; Changsong Wang; Yulei Gong; Ying He |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of anaesthesiology Volume: 26 ISSN: 1365-2346 ISO Abbreviation: Eur J Anaesthesiol Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-05-14 Completed Date: 2009-08-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8411711 Medline TA: Eur J Anaesthesiol Country: England |
Other Details:
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Languages: eng Pagination: 513-9 Citation Subset: IM |
Affiliation:
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Department of Anesthesiology, The First Affiliated Hospital of Harbin Medical University, Harbin, P.R. China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aspartate Aminotransferases / blood Biological Markers / analysis Breath Tests Lipid Peroxidation* Liver Diseases / metabolism* Male Malondialdehyde / blood Monitoring, Physiologic Pentanes / analysis* Reperfusion Injury / metabolism* Swine |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Pentanes; 542-78-9/Malondialdehyde; EC 2.6.1.1/Aspartate Aminotransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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